Effect of excessive cholesterol and lipopolysaccharide on cerebellar neuronal cells in in vitro and protective role of anti-inflammatory drugs.

The present work was carried out to elucidate the role of NSAIDs, PPARg agonist and HMG CoA inhibitor on cholesterol and lipopolysaccharide (LPS) induced neurodegeneration. The cerebellar, neuronal cells were exposed to cholesterol (10 and 50 microg/ml), LPS (1 ng/ml) or both. Neuroprotective effect of ibuprofen, rofecoxib, simvastatin and pioglitazone was assessed by measuring the neuronal loss, MTT dye assay, nitric oxide, LDH and lipid peroxide measurement.