Double-blind, randomized comparison of the effect of carbetocin and oxytocin on intraoperative blood loss and uterine tone of patients undergoing cesarean section.

Objectives: A double-blind randomized study involving pregnant women undergoing cesarean section was conducted to compare the effectiveness of a single 100 micrograms intravenous injection of the long-acting oxytocin analog, carbetocin, with that of a standard infusion of oxytocin with respect to intraoperative blood loss. The two treatments also were compared for safety and ability to maintain adequate uterine tone.

Study Design: The study drug was administered to 57 women during elective cesarean section after placental delivery; blood was collected until abdominal closure.

Epitope mapping of human monoclonal antibodies to HLA-B27 by using natural and mutated antigenic variants.

The epitopes defined by three human monoclonal antibodies (mAbs) (Tr3B6, TrCG10, TrBH12) against HLA-B27 have been mapped by flow cytometry. For this purpose we used murine transfected cells expressing at their surface hybrid antigens between HLA-B7 and -B27 and, in addition, Epstein-Barr virus cell lines expressing the six HLA-B27 alleles B*2701 to B*2706. The results indicated that the mAbs are domain specific.

The hepatic chalone. II. Chemical and biological properties of the rabbit liver chalone.

A factor has been purified from rabbit liver which decreases the incorporation of 3H-thymidine in DNA of regenerating rat liver slices. This effect is due mostly to an inhibition of DNA synthesis from the deoxynucleoside triphosphates. The purified rabbit liver factor thus interferes with the liver cell division cycle.

Inhibition of cell proliferation in the livers of hepatectomized rats by a rabbit hepatic chalone.

A purified chalone isolated from rabbit liver was tested in vitro on regenerating rat liver slices incubated with tritiated thymidine to determine more precisely the phase of the normal cell cycle that was blocked by that substance. Biochemical and radioautographic studies showed that the inhibition of tritiated thymidine incorporation during chromosomal DNA replication resulted chiefly from a block in the G(1)-S transition in the normal cell cycle. Under these conditions the chalone had little inhibitory effect on hepatocytes that were in the S phase of the cell cycle.