Brucellosis is a serious infectious disease that causes significant economic losses in the livestock industry. Its early diagnosis allows an adequate disease control in cattle. DAVIH Laboratories designed a lateral flow immunochromatographic assay using protein A-colloidal gold as a detector reagent (LFIA-PA).
View Article and Find Full Text PDFIntroduction: antigen immobilization in solid supports is used for the development of several immunoassays. One of the first technologies developed was the protein adsorption by direct application to nitrocellulose.
Objective: to standardize the immobilization of a synthetic peptide of the HIV-2 transmembrane protein gp36 to nitrocellulose support for diagnostic purposes and to evaluate the performance parameters in a group of serum samples with recognized interesting reactivity.