High-throughput FastCloning technology: A low-cost method for parallel cloning.

FastCloning, a reliable cloning technique for plasmid construction, is a widely used protocol in biomedical research laboratories. Only two-step molecular manipulations are required to add a gene (cDNA) of interest into the desired vector. However, parallel cloning of the gene into multiple vectors is still a labor-intensive operation, which requires a range of primers for different vectors in high-throughput cloning projects.

Finite Element Analysis of the Mechanical Properties of Axially Compressed Square High-Strength Concrete-Filled Steel Tube Stub Columns Based on a Constitutive Model for High-Strength Materials.

With the development of new concrete technology, high-strength concrete has been used worldwide. In particular, more economic benefits can be achieved by applying high-strength concrete-filled steel tube (HSCFST) columns in the concrete core walls of super high-rise buildings. A constitutive relation with high applicability for high-strength materials with different strength grades is proposed.

A Unique Homo-Hexameric Structure of 2-Aminomuconate Deaminase in the Bacterium .

The bacterium sp. is one of several microorganisms that are capable of using 2-aminophenol as its sole source of carbon, nitrogen and energy. Several 2-aminophenol-metabolizing enzymes have pivotal roles in the biodegradation of aniline and its derivatives as environmental pollutants in .

Structural basis for the acetylation of histone H3K9 and H3K27 mediated by the histone chaperone Vps75 in .

Rtt109 is a histone acetyltransferase (HAT) that is a potential therapeutic target in conditioned pathogenic fungi . The histone chaperone Vps75 can stimulate the Rtt109-dependent acetylation of several histone H3 lysines and preferentially acetylates H3K9 and H3K27 within canonical histone (H3-H4) tetramers. Vps75 shows two protein conformations assembled into dimeric and tetrameric forms, but the roles played by multimeric forms of Vps75 in Rtt109-mediated histone acetylation remain elusive.

Structural features and kinetic characterization of alanine racemase from Bacillus pseudofirmus OF4.

Alanine racemase (Alr) is a pyridoxal-5'-phosphate-dependent (PLP) enzyme that catalyzes a reversible racemization between the enantiomers of alanine. d-Alanine is an indispensable constituent in the biosynthesis of bacterial cell-wall peptidoglycan, and its inhibition is lethal to prokaryotes, which makes it an attractive target for designing antibacterial drugs. In this study, the molecular structure of alanine racemase from Bacillus pseudofirmus OF4 (DadX) was determined by X-ray crystallography to a resolution of 1.

Structural basis for dimerization and RNA binding of avian infectious bronchitis virus nsp9.

The potential for infection by coronaviruses (CoVs) has become a serious concern with the recent emergence of Middle East respiratory syndrome and severe acute respiratory syndrome (SARS) in the human population. CoVs encode two large polyproteins, which are then processed into 15-16 nonstructural proteins (nsps) that make significant contributions to viral replication and transcription by assembling the RNA replicase complex. Among them, nsp9 plays an essential role in viral replication by forming a homodimer that binds single-stranded RNA.