The Quantitative Assessment of (PA)14 Biofilm Surface Coverage on Slippery Liquid Infused Polymer Surfaces (SLIPS).

Slippery, porous polymeric antimicrobial surfaces for biofilm attachment inhibition of the clinical strain (PA14) have been prepared. Porous BMA-EDMA, characterized for its hydrophobic properties, was infused with a slippery liquid creating a hydrophobic liquid interface and characterized by water contact angle and SEM. A low shear force bioreactor was used to prepare biofilms on these antimicrobial surfaces.

Using Fluorescence Intensity of Enhanced Green Fluorescent Protein to Quantify .

A variety of direct and indirect methods have been used to quantify planktonic and biofilm bacterial cells. Direct counting methods to determine the total number of cells include plate counts, microscopic cell counts, Coulter cell counting, flow cytometry, and fluorescence microscopy. However, indirect methods are often used to supplement direct cell counting, as they are often more convenient, less time-consuming, and require less material, while providing a number that can be related to the direct cell count.