Zinc-dependent activation of the Pho8 alkaline phosphatase in .

Genome-wide analyses have revealed that during metal ion starvation, many cells undergo programmed changes in their transcriptome or proteome that lower the levels of abundant metalloproteins, conserving metal ions for more critical functions. Here we investigated how changes in cellular zinc status affect the expression and activity of the zinc-requiring Pho8 alkaline phosphatase from fission yeast (). In , Pho8 is a membrane-tethered and processed glycoprotein that resides in the vacuole.