Studying the protein expression in human B lymphoblastoid cells exposed to 1.8-GHz (GSM) radiofrequency radiation (RFR) with protein microarray.

In the present study, the protein microarray was used to investigate the protein expression in human B-cell lymphoblastoid cells intermittently exposed to 1.8-GHz GSM radiofrequency radiation (RFR) at the specific absorption rate (SAR) of 2.0 W/kg for 24 h.

Superposition of an incoherent magnetic field inhibited EGF receptor clustering and phosphorylation induced by a 1.8 GHz pulse-modulated radiofrequency radiation.

Purpose: The present study was conducted to investigate the effect of a temporally incoherent ('noise') magnetic field (MF) on radiofrequency radiation (RFR)-induced epidermal growth factor (EGF) receptor clustering and phosporylation in cultured cells.

Materials And Methods: Human amniotic epithelial (FL) cells were exposed for 15 min to either a 1.8 GHz RFR (modulated at 217 Hz), a 2 μT incoherent MF, or concurrently to the RFR and incoherent MF.

Using model organism Saccharomyces cerevisiae to evaluate the effects of ELF-MF and RF-EMF exposure on global gene expression.

The potential health hazard of exposure to electromagnetic fields (EMF) continues to cause public concern. However, the possibility of biological and health effects of exposure to EMF remains controversial and their biophysical mechanisms are unknown. In the present study, we used Saccharomyces cerevisiae to identify genes responding to extremely low frequency magnetic fields (ELF-MF) and to radiofrequency EMF (RF-EMF) exposures.

A 1.8-GHz radiofrequency radiation induces EGF receptor clustering and phosphorylation in cultured human amniotic (FL) cells.

Purpose: Many studies have shown that exposure to radiofrequency radiation (RFR) could activate cellular signal transduction pathways. In the present research, we investigated the effects of exposure to a 1.8-GHz RFR at different intensities on epidermal growth factor (EGF) receptor clustering and phosphorylation in human amniotic (FL) cells.

Superimposition of an incoherent magnetic field eliminated the inhibition of hormone secretion induced by a 50-Hz magnetic field in human villous trophoblasts in vitro.

The effects of exposure to a sinusoidal 50-Hz magnetic field (MF) and an incoherent (noise) MF on hormone secretion in human first trimester villous trophoblasts were investigated. Trophoblasts were isolated from more than 30 first trimester human chorionic villi of 8-10 weeks gestation. They were cultured and exposed to MFs for different durations.

[A preliminary study on role of acid sphingomyelinase in receptor clustering induced by 50-Hz magnetic fields].

Objective: To investigate the relationship among a 50-Hz MF-induced epidermal growth factor receptor (EGFR) clustering, acid sphingomyelinase (A-SMase) and ceramide (CER), and to explore the possible mechanism of receptor clustering.

Methods: Human amnion (FL) cells were exposed to a 50-Hz sinusoidal magnetic field at 0.4 mT for 15 min with or without imipramine, a specific inhibitor of A-SMase and ceramide pretreatment.

Impact of 1.8-GHz radiofrequency radiation (RFR) on DNA damage and repair induced by doxorubicin in human B-cell lymphoblastoid cells.

In the present in vitro study, a comet assay was used to determine whether 1.8-GHz radiofrequency radiation (RFR, SAR of 2W/kg) can influence DNA repair in human B-cell lymphoblastoid cells exposed to doxorubicin (DOX) at the doses of 0microg/ml, 0.05microg/ml, 0.

Influence of 1.8-GHz (GSM) radiofrequency radiation (RFR) on DNA damage and repair induced by X-rays in human leukocytes in vitro.

In the present study, the in vitro comet assay was used to determine whether 1.8-GHz radiofrequency radiation (RFR) can influence DNA repair in human leukocytes exposed to X-rays. The specific energy absorption rate (SAR) of 2 W/kg (the current European safety limit) was applied.

[Effect of 1.8 GHz radiofrequency electromagnetic fields on gene expression of rat neurons].

Objective: To investigate the changes of gene expression in rat neuron induced by 1.8 GHz radiofrequency electromagnetic fields (RF EMF) to screen for RF EMF-responsive genes and the effect of different exposure times and modes on the gene expression in neuron.

Methods: Total RNA was extracted immediately and purified from the primary culture of neurons after intermittent exposed or sham-exposed to a frequency of 1.

An incoherent magnetic field inhibited EGF receptor clustering and phosphorylation induced by a 50-Hz magnetic field in cultured FL cells.

Previously, we found that exposure to a 50-Hz magnetic field (MF) at 0.4 mT could induce epidermal growth factor (EGF) receptor clustering in Chinese hamster lung (CHL) fibroblast cells and superposition of an incoherent MF with the same intensity could inhibit the effect. In the present experiment, we investigated the effects of 50-Hz MF exposure at different intensities on EGF receptor clustering and phosphorylation in human amniotic cells (FL), and explored the interaction effect of an incoherent MF.

Effect of superposed electromagnetic noise on DNA damage of lens epithelial cells induced by microwave radiation.

Purpose: To investigate the influence of the 1.8-GHz radiofrequency fields (RFs) of the Global System for Mobile Communications on DNA damage, intracellular reactive oxygen species (ROS) formation, cell cycle, and apoptosis in human lens epithelial cells (hLECs) and whether the effects induced by RF could be blocked by superposing of electromagnetic noise.

Methods: After 24-hour intermittent exposure at the specific absorption rate of 1 W/kg, 2 W/kg, 3 W/kg, and 4 W/kg, the DNA damage of hLECs was examined by alkaline comet assay and immunofluorescence microscope detection of the phosphorylated form of histone variant H2AX (gammaH2AX) foci, respectively.

[Blocking 1800 MHz mobile phone radiation-induced reactive oxygen species production and DNA damage in lens epithelial cells by noise magnetic fields].

Objective: To investigate whether the exposure to the electromagnetic noise can block reactive oxygen species (ROS) production and DNA damage of lens epithelial cells induced by 1800 MHz mobile phone radiation.

Methods: The DCFH-DA method and comet assay were used respectively to detect the intracellular ROS and DNA damage of cultured human lens epithelial cells induced by 4 W/kg 1800 MHz mobile phone radiation or/and 2 muT electromagnetic noise for 24 h intermittently.

Result: 1800 MHz mobile phone radiation at 4 W/kg for 24 h increased intracellular ROS and DNA damage significantly (P<0.

[Surface markers and functions of human dendritic cells exposed to mobile phone 1800 MHz electromagnetic fields].

Objective: To investigate the effects of mobile phone 1800 MHz electromagnetic fields (EMF) on the surface markers and the functions of human dendritic cells (DC).

Methods: Human DCs were exposed to intermittent 5 min on/10 min off EMF with specific absorption rates (SAR) 4 W/kg for 0 h, 1 h, 12 h or 24 h, respectively. FACS analysis was used to detect the positive percentage of DC surface markers including HLA-DR and co-stimulatory molecules such as CD80, CD86, CD40 and CD11c.

[Effects of millimeter wave on gene expression in human keratinocytes].

Objective: To explore the effect of millimeter wave exposure at low power density on gene expression in human keratinocytes (HaCaT).

Methods: HaCaT keratinocytes were exposed to 30.16 GHz millimeter wave with power densities of 1.

[Effects of 50 Hz magnetic fields on gene expression in MCF-7 cells].

Objective: To investigate whether 50 Hz magnetic fields (MF) can change the gene expression profile in MCF-7 cells and to screen MF responsive genes.

Methods: In vitro cultured MCF-7 cells were continuously exposed or sham-exposed to 0.4 mT of 50 Hz MF for 24 hours.

[Effects of 50 Hz magnetic fields on DNA double-strand breaks in human lens epithelial cells].

Objective: To investigate the effects of 50 Hz magnetic fields (MF) on DNA double-strand breaks in human lens epithelial cells (hLECs).

Methods: The cultured human lens epithelial cells were exposed to 0.4 mT 50 Hz MF for 2 h, 6 h, 12 h, 24 h and 48 h.

[Preliminary study on role of lipid rafts in receptor clustering induced by 50 Hz magnetic fields and its mechanism].

Objective: To investigate the relationship among a 50 Hz magnetic field (MF)-induced epidermal growth factor receptor (EGFR) clustering,lipid rafts and acid sphingomyelinase (ASM), and to explore its possible mechanism.

Methods: Human amnion FL cells were exposed to 50 Hz, 0.4 mT MF for 15 min.

Proteomic analysis of human lens epithelial cells exposed to microwaves.

Purpose: To study proteomic changes in human lens epithelial cells (HLECs) exposed to 1800-MHz Global System for Mobile Communication (GSM)-like microwaves.

Methods: In three separate experiments, HLECs were exposed and sham-exposed (six dishes each) to 1800-MHz GSM-like radiation for 2 h. The specific absorption rates were 1.

[Effects of 50 Hz magnetic fields exposure on secretion of primary human villous trophoblasts].

Objective: To explore the possible effects of 50 Hz magnetic fields (MF) exposure on HCG and progesterone secretion of human villous trophoblasts in vitro.

Methods: The trophoblasts were isolated from human villus by trypsin digestion and incubated in DMEM medium. Then the trophoblasts were exposed to 0.

[Influence of 1.8 GHz microwave on DNA damage induced by ultraviolet C ray].

Objective: To study whether 1.8 GHz microwaves (MW) (SAR, 3 W/kg) exposure can influence DNA damage induced by ultraviolet ray (UV).

Methods: The lymphocytes were obtained from three young healthy donors.

[Effects of microwave radiation on lens hydration and expression of PKC-alpha and transcription factors in lens epithelial cells].

Objective: To observe the effects of low power microwave radiation on lens hydration and lens epithelial cells in vitro, and detect the expression of PKC-alpha, c-fos and c-jun in lens epithelial cells.

Methods: Rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.5, 2.

Absence of effect of power-frequency magnetic fields exposure on mouse embryonic lens development.

There are no reports in the literature investigating the biological effect of power-frequency (50/60 Hz) magnetic fields on embryonic lens development. We investigated whether power-frequency magnetic fields act as an environmental insult and induce changes in morphology or protein and/or transcription factor mRNA expression levels in developing mouse embryonic lenses. Three groups of pregnant mice were exposed to magnetic fields (50 Hz) of varying intensities (0.

[Effects of different dose microwave radiation on protein components of cultured rabbit lens].

Objective: To study the effects of different dose microwave radiation on protein components of cultured rabbit lens, and analyze the mechanisms of lens injury caused by microwave radiation.

Methods: Cultured rabbit lens were exposed to microwave radiation with frequency of 2450 MHz and power density of 0.25, 0.

Studying gene expression profile of rat neuron exposed to 1800MHz radiofrequency electromagnetic fields with cDNA microassay.

A widespread use of mobile phone (MP) evokes a growing concern for their possible adverse effects on human, especially the brain. Gene expression is a unique way of characterizing how cells and organism adapt to changes in the external environment, so the aim of this investigation was to determine whether 1800 MHz radiofrequency electromagnetic fields (RF EMF) can influence the gene expression of neuron. Affymetrix Rat Neurobiology U34 array was applied to investigate the changes of gene expression in rat neuron after exposed to the pulsed RF EMF at a frequency of 1800 MHz modulated by 217 Hz which is commonly used in MP.