Spatiotemporal information enhanced multi-feature short-term traffic flow prediction.

Accurately predicting traffic flow is crucial for optimizing traffic conditions, reducing congestion, and improving travel efficiency. To explore spatiotemporal characteristics of traffic flow in depth, this study proposes the MFSTBiSGAT model. The MFSTBiSGAT model leverages graph attention networks to extract dynamic spatial features from complex road networks, and utilizes bidirectional long short-term memory networks to capture temporal correlations from both past and future time perspectives.

A Lightweight Vehicle Detection Method Fusing GSConv and Coordinate Attention Mechanism.

Aiming at the problems of target detection models in traffic scenarios including a large number of parameters, heavy computational burden, and high application cost, this paper introduces an enhanced lightweight real-time detection algorithm, which exhibits higher detection speed and accuracy for vehicle detection. This paper considers the YOLOv7 algorithm as the benchmark model, designs a lightweight backbone network, and uses the MobileNetV3 lightweight network to extract target features. Inspired by the structure of SPPF, the spatial pyramid pooling module is reconfigured by incorporating GSConv, and a lightweight SPPFCSPC-GS module is designed, aiming to minimize the quantity of model parameters and enhance the training speed even further.

A novel adenoviral vector-mediated neuronal selective gene expression in neonatal mouse brain in response to hypoxia.

Selective gene expression targeting neurons is a challenge, which, if successfully overcome, carries an enormous potential for clinical applications in therapeutics against neurodegenerative diseases. We have reported previously the construction of a series of adenoviral vectors capable of selectively expressing a reporter gene luciferase in cultured neurons [D. Huang, A.

Calpain-cleaved collapsin response mediator protein-3 induces neuronal death after glutamate toxicity and cerebral ischemia.

Collapsin response mediator proteins (CRMPs) mediate growth cone collapse during development, but their roles in adult brains are not clear. Here we report the findings that the full-length CRMP-3 (p63) is a direct target of calpain that cleaves CRMP-3 at the N terminus (+76 amino acid). Interestingly, activated calpain in response to excitotoxicity in vitro and cerebral ischemia in vivo also cleaved CRMP-3, and the cleavage product of CRMP-3 (p54) underwent nuclear translocation during neuronal death.

The gas7 protein potentiates NGF-mediated differentiation of PC12 cells.

The growth-arrest-specific protein gas7 is required for morphological differentiation of cultured mouse cerebellar neurons and PC12 cells. Moreover, its overexpression in various cell types induces neurite-like outgrowth. The role of gas7 in neuronal differentiation was further characterized by adenovirus-mediated overexpression in PC12 cells and quantification of the expression of various neuronal markers, in the absence and presence of different concentrations of nerve growth factor (NGF).

Characterization of the expression of key adenoviral receptors CAR and integrin beta3/beta5 subunits on the membrane of human NT2 neurons.

Expression of therapeutic gene products in differentiated human NT2 neurons (NT2/Ns) is being explored for ex vivo gene therapy of human neurological diseases. In this study we determined the efficiency of adenovirus (Ad)-mediated gene delivery into NT2/Ns and characterized the expression of several key receptors known to be required for efficient Ad-mediated gene delivery. Undifferentiated NT2 cells and NT2/Ns were infected by Ad expressing green fluorescent protein at an efficiency of 33% and 17%, respectively percentages much lower than the 92% infectivity obtained from a human non-neuronal cell line A549 cells.