MPXV: Update on Morphological and Morphogenesis Aspects Through Transmission and Scanning Electron Microscopies and 3D Reconstruction.

An unprecedented global outbreak caused by the monkeypox virus (MPXV) prompted the World Health Organization to declare a public health emergency of international concern on July 23, 2022. Therapeutics and vaccines for MPXV are not widely available, necessitating further studies, particularly in drug repurposing area. To this end, the standardization of in vitro infection systems is essential.

The Effects of Hydrological Disasters on the Population's Health in the Northeast Region of Brazil.

The Northeast region of Brazil is characterized by long periods of drought. However, the region is also frequently affected by floods. The socioeconomic characteristics of the locality make the population more vulnerable to the impacts of these disasters.

Implementation of a Laboratory Information Management System (LIMS) for microbiology in Timor-Leste: challenges, mitigation strategies, and end-user experiences.

Background: Effective diagnostic capacity is crucial for clinical decision-making, with up to 70% of decisions in high-resource settings based on laboratory test results. However, in low- and middle-income countries (LMIC) access to diagnostic services is often limited due to the absence of Laboratory Information Management Systems (LIMS). LIMS streamline laboratory operations by automating sample handling, analysis, and reporting, leading to improved quality and faster results.

Selective activation of FZD2 and FZD7 reveals non-redundant function during mesoderm differentiation.

During gastrulation, Wnt-β-catenin signaling dictates lineage bifurcation generating different mesoderm cell types. However, the specific role of Wnt receptors in mesoderm specification remains elusive. Using selective Frizzled (FZD) and LRP5/6 antibody-based agonists, we examined FZD receptors' function during directed mesoderm differentiation of human pluripotent stem cells (hPSCs).

BCAA interactions: how do they influence broiler performance, intestinal morphometry, lipid profile, and liver health?

An experiment was conducted to assess the effects of the BCAA and their interactions on performance, carcass composition, lipid metabolism, liver health, and intestinal morphometry in broiler chickens. Male chickens ( = 1080) were randomly assigned into floor pens in a 3 × 3 factorial design with 3 dietary ratios of SID Leu:Lys (110, 150, and 190%), and 3 dietary ratios of SID Ile-Val:Lys (68-77, 78-87, and 88-97%). Performance parameters were assessed from 1 to 35 days of age.

Untreated women with first trimester fasting glycaemia 92-125 mg/dL and risk of gestational diabetes mellitus in the 24-28th week OGTT: prevalence and predictors.

Introduction: Women with first trimester fasting glycaemia (FTFG) 92-125 mg/dL may present with normal 24-28th week OGTT (2T-OGTT). Predictors of persistent hyperglycaemia were scarcely investigated. We studied the prevalence and predictors of gestational diabetes mellitus (GDM) in the 2T-OGTT in women with untreated elevated FTFG.

Development of chitosan-based films with invertase enzyme for active packaging: Physicochemical parameters, structure, application, and storage.

The use of active packaging made from biodegradable polymers can contribute to the environment and to the food industry by increasing the shelf life of their products. This study aimed to produce chitosan-based films incorporated with the invertase enzyme (1, 2, 5, 9, and 10 %) as an alternative to avoid sucrose crystallization in the confectionery industry. The optimum activity of the invertase enzyme was observed at 55 °C and pH 5, thus, the films made with the film-forming solution adjusted to pH 5 and dried at 55 °C were compared with those without pH adjustment and dried at room temperature.

Surface coating nanoarchitectonics for optimizing cytocompatibility and antimicrobial activity: The impact of hyaluronic acid positioning as the outermost layer.

Polyelectrolyte multilayers (PEMs) based on hyaluronic acid (HA) and poly (diallyldimethylammonium chloride) (PDDA) were deposited on oxidized polystyrene (PS) via the layer-by-layer (LbL) method. The X-ray photoelectron spectroscopy (XPS) confirmed the PEM deposition on PS, and atomic force microscopy (AFM) indicated that the surface roughness of PS also increased after PEM deposition. The PEMs significantly enhanced PS wettability, reducing the contact angle from 73° on PS to 24° on PDDA-terminated (PDDA/HA) PEM (2.

Mineralocorticoid Effects in Cushing's Disease: A Case Report.

Cushing's syndrome is a rare disease caused due to prolonged exposure to excess glucocorticoids. Although rare, diagnosing Cushing's syndrome is clinically significant as it allows tailored and timely management and significant reduction or even prevention of the comorbidities caused by cortisol excess. This report delineates the presentation of a 44-year-old female with refractory secondary hypertension and severe hypokalaemia, initially thought to be caused by hyperaldosteronism.

Buthionine sulfoximine acts synergistically with doxorubicin as a sensitizer molecule on different tumor cell lines.

The chemotherapeutic drug doxorubicin (DOX) has been widely used for treating solid tumors attributed to its antiproliferative effectiveness; however, its clinical use is limited due to side effects, including cardiotoxicity, myelosuppression, and drug resistance. Combining DOX with buthionine sulfoximine (BSO), a glutathione (GSH) synthesis inhibitor, showed promising results in overcoming these adverse effects, potentially reducing the required DOX dose while maintaining efficacy. The aim of the present study was to examine the effects of different concentrations of BSO and DOX, both individually and in combination, utilizing B16/F10 (murine melanoma), SNB-19 (human glioblastoma), S180 (murine sarcoma), and SVEC4-10 (murine endothelial) cell lines.

Artificial intelligence as a transforming factor in motility disorders-automatic detection of motility patterns in high-resolution anorectal manometry.

High-resolution anorectal manometry (HR-ARM) is the gold standard for anorectal functional disorders' evaluation, despite being limited by its accessibility and complex data analysis. The London Protocol and Classification were developed to standardize anorectal motility patterns classification. This proof-of-concept study aims to develop and validate an artificial intelligence model for identification and differentiation of disorders of anal tone and contractility in HR-ARM.

Structure-based Virtual Screening and Drug Design Development of Leishmanicidal Pyrimidines.

Leishmaniasis is a neglected disease caused by parasites of the genus Leishmania sp. that causes approximately 1 million cases and 650,000 deaths annually worldwide. Its treatment has several limitations mainly due to high toxicity and clinical resistance, and the search for alternatives is highly desirable.

Efficacy and safety of intravenous tirofiban in patients with acute ischemic stroke due to large artery atherosclerosis undergoing endovascular thrombectomy: A systematic review and meta-analysis.

Introduction: Intravenous tirofiban (IT) is shown to be potentially effective in acute ischemic stroke (AIS) patients submitted to mechanical thrombectomy, despite its safety and efficacy are not well established. However, there is a lack of evidence on the effects of IT on endovascular thrombectomy (EVT) in patients with AIS due to large artery atherosclerosis (LAA).

Objectives: To assess the safety and efficacy of IT in AIS patients due to LAA submitted to EVT.

Social network for guardians of transgender children and adolescents.

Objective: To analyze the social network of mothers, fathers or guardians of transgender children or adolescents.

Methods: This was a qualitative study, based on the theoretical framework of social network, with a focus on the primary network. The study was conducted in Brazil through online interviews between August and October 2021.

Clinical simulation training for first responders in pediatric emergencies with family interaction: a scoping review.

Objective: To map studies on clinical simulation training directed at first responders during pediatric emergencies, focusing on interaction with families.

Methods: A scoping review based on the guidelines of the JBI Manual for Evidence Syntheses and reported according to the PRISMA-ScR checklist, covering eight databases and gray literature, without time or language restrictions.

Results: The ten selected studies indicated that most publications were from the United States.

Construction of a bundle for the safety of patients with mental disorders during hospitalization.

Objective: to develop a bundle for the safety of psychiatric patients during hospitalization.

Methods: a methodological study conducted in two stages. In the first, a comprehensive literature review was developed through a scoping review and conducted to examine evidence on the safety of psychiatric patients during hospitalization.

Unfolded protein response during the progression of colorectal carcinogenesis.

Purpose: To evaluate the molecular evolution of endoplasmic reticulum (ER) stress during colorectal cancer carcinogenesis.

Methods: Fifty-six hairless mice were divided into two groups: control (no intervention); and carcinogenesis (treated with two doses of azoxymethane at 10 mg/kg during the third and the fourth week and dextran sodium sulfate at 2.5% for seven days in the second, fifth, and eighth week).

Abortion-related complications in Brazil: results from the World Health Organization Multi-country Survey on Abortion (MCS-A).

This study aimed to describe the severity of abortion-related complications, factors associated with complications, the types of management and the experience of care in Brazil. A cross-sectional study in twenty hospitals (10 in Federal District, 3 in Rondônia and 7 in Maranhão). For 3 months, all women treated for abortion/miscarriage had their data collected.

Essential oil of Lippia origanoides as an anesthetic for Piaractus mesopotamicus: implications for induction and recovery times, ventilatory frequency and blood responses after biometric management.

This study evaluated the use of the essential oil of Lippia origanoides (EOLO) as an anesthetic for juvenile pacu, Piaractus mesopotamicus. Two experiments were performed. In Experiment I, anesthetic induction and recovery times and ventilatory frequency (VF) were determined for fish (n= 48; 29.

The rehabilitation of children with brain tumors using exergames: a scoping review protocol.

Objective: To map the literature on the use of exergames in the rehabilitation of school-age children with brain tumors, in any context.

Method: Scoping review protocol developed using the recommendations of the Joanna Briggs Institute. The search will include aggregators, databases, indexes, repositories, and research browsers, without limitation as to the year of publication.

regulates melanocortin 4 receptor transcription and energy homeostasis.

Disruption of hypothalamic melanocortin 4 receptors (MC4Rs) causes obesity in mice and humans. Here, we investigated the transcriptional regulation of in the hypothalamus. In mice, we show that the homeodomain transcription factor Orthopedia (OTP) is enriched in MC4R neurons in the paraventricular nucleus (PVN) of the hypothalamus and directly regulates transcription.

Effects of using microalgae in poultry diets on the production and quality of meat and eggs: a review.

1. This review was conducted to examine the nutritional composition of microalgae and their effects as a feed ingredient in poultry diets, delving into their influence on the production and quality of meat and eggs. Data collection focused on peer-reviewed scientific articles, with no limitation on the temporal horizon.

Dynamic Metabolomic Changes in the Phenolic Compound Profile and Antioxidant Activity in Developmental Sorghum Grains.

Phenolic compounds (PC) were analyzed by UHPLC-ESI-QTOF-MS in two sorghum genotypes, harvested in two growing seasons (GS) at five distinct days after flowering (DAF) to evaluate how genotype/GS influences the PC synthesis and antioxidant capacity during grain growth. Total phenolic contents were strongly correlated with antioxidant capacity ( > 0.9, < 0.

Balance Changes in Adult Cancer Survivors Participating in a 16-Week Therapeutic Yoga Program.

Introduction: Balance problems arising from cancer and its treatments can significantly impact daily functionality and quality of life. Improving balance as part of a cancer treatment plan could result in better patient outcomes. Thus, the aim of this study was to determine whether an integrative therapeutic yoga intervention can improve balance in a heterogenous population of cancer survivors (CS).