Can insecticide applications used to kill vector insects prevent pine wilt disease?

Background: The aerial application of insecticides is a primary method used to prevent the spread of pine wilt disease by reducing the population density of Monochamus beetles, the vector insects of pine wood nematodes (PWNs). This study investigated the mortality of vector insects and the ratio of PWN-infected trees according to systemically remaining thiacloprid residues in Pinus densiflora. To do this, thiacloprid was sprayed on a nursery of 5-year-old P.

Point-of-care diagnostic (POCD) method for detecting Bursaphelenchus xylophilus in pinewood using recombinase polymerase amplification (RPA) with the portable optical isothermal device (POID).

The pinewood nematode (PWN), Bursaphelenchus xylophilus, is a causative agent of pine wilt disease (PWD). To date, although several molecular diagnostic methods have been developed, rapid on-site diagnostic tools for detecting PWN in pinewood are limited. In this study, a point of care diagnostic (POCD) method for detecting PWN in pinewood using recombinase polymerase amplification (RPA) assay was developed.