Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of the Operon.

Using reporter gene () transcriptional fusions we examined the transcriptional dependencies of the promoter (P) and the entire operon regulatory region (P-) on eight transcription factors as well as the inducer, salicin, and an IS5 insertion upstream of P. Crp-cAMP is the primary activator of both P and the operon, while H-NS is a strong dominant operon repressor but only a weak repressor of P. H-NS may exert its repressive effect by looping the DNA at two binding sites.

Insertion Sequence (IS) Element-Mediated Activating Mutations of the Cryptic Aromatic β-Glucoside Utilization () Operon Are Promoted by the Anti-Terminator Protein (BglG) in .

The cryptic β-glucoside GFB () operon in () can be activated by mutations arising under starvation conditions in the presence of an aromatic β-glucoside. This may involve the insertion of an insertion sequence (IS) element into a "stress-induced DNA duplex destabilization" (SIDD) region upstream of the operon promoter, although other types of mutations can also activate the operon. Here, we show that increased expression of the gene, encoding a well-characterized transcriptional antiterminator, dramatically increases the frequency of both IS-mediated and IS-independent Bgl mutations occurring on salicin- and arbutin-containing agar plates.

Application of spin-ratio scaled MP2 for the prediction of intermolecular interactions in chemical systems.

Accurate prediction of intermolecular interactions plays a pivotal role in many areas of chemistry and biology including (but not limited to) the design of pharmaceuticals, solid electrolytes and food additives. Here we present the application of the recently developed spin-ratio scaled MP2 method (termed SRS-MP2) to six different datasets covering a wide range of interaction types from strong hydrogen bonding to van der Waals dispersion and π-π stacking. The method achieves a remarkably low mean absolute error of 1.

Gait Training in Patients Discharged to a Skilled Nursing Facility Following Total Joint Arthroplasty.

Background: Expenditures for postacute care in total joint arthroplasty (TJA) have risen dramatically over recent decades. Therefore, efforts are underway to better identify cost savings in postacute rehabilitation centers, such as skilled nursing facilities (SNFs). The primary purpose of this study was to analyze gait training achievements in post-TJA patients in the interval between hospital discharge and the patients' first 4 days at the SNF.

Comparative Analysis of Calcium Silicate-based Root Filling Materials Using an Open Apex Model.

Introduction: Many new calcium silicate-based root filling materials have emerged in the market; however, their performance in the orthograde obturation of an open apex has not been evaluated. The purpose of this study was to compare the marginal adaptation of ProRoot MTA (Dentsply Tulsa Dental, Tulsa, OK), NeoMTA Plus (Avalon Biomed Inc, Bradenton, FL), and Endosequence BC RRM-Fast Set Putty (BC RRM-FS; Brasseler USA, Savannah, GA) after orthograde placement in roots with open apices.

Methods: Palatal roots of maxillary molars were instrumented to create divergent open apices and divided into 4 groups for orthograde obturation: ProRoot MTA, NeoMTA Plus, BC RRM-FS, and BC RRM-FS + BC Sealer.

Nuclear magnetic resonance methods for metabolic fluxomics.

Fluxomics, through its core methodology of metabolic flux analysis (MFA), enables quantification of carbon traffic through cellular biochemical pathways. Isotope labeling experiments aid MFA by providing information on intracellular fluxes, especially through parallel and cyclic pathways. Nuclear magnetic resonance (NMR) and mass spectrometry (MS) are two complementary methods to measure abundances of isotopomers generated in these experiments.