Mitochondrial bioenergetic profiles of warmed bovine blastocysts are typically altered after cryopreservation by slow freezing and vitrification.

The widespread use of cryopreserved in vitro produced (IVP) bovine embryos is limited due to their low post-warming viability compared to their ex vivo derived counterparts. Therefore, the present study aimed to analyse in detail the consequences of cryopreservation (vitrification and slow freezing) on the bioenergetic profile of the embryo and its mitochondria. To accomplish that, day 7 IVP embryos were separated in a non-cryopreserved control group (fresh, n = 120, 12 replicates) or were either slow frozen (slow frozen, n = 60, 6 replicates) or vitrified (vitrified, n = 60, 6 replicates).

The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events.

At the embryonic level, CRISPR technologies have been used to edit genomes reliably and efficiently in various mammalian models, with Ribonucleoprotein (RNP) electroporation potentially representing a superior delivery method into mammalian zygotes. However, detailed insights of the interactions between varying technical settings as well as the time point of electroporation in a bovine zygote's cell cycle on developmental metrics and the frequency and type of editing events are largely unknown. The present study uncovers that increasing pulse lengths result in higher Full Edit rates, with Mosaicism in Full-Edit embryos being significantly affected by adjusting RNP-electroporation relative to zygote cell cycle.

The global gene expression outline of the bovine blastocyst: reflector of environmental conditions and predictor of developmental capacity.

Background: Morphological evaluation of embryos has been used to screen embryos for transfer. However, the repeatability and accuracy of this method remains low. Thus, evaluation of an embryo's gene expression signature with respect to its developmental capacity could provide new opportunities for embryo selection.

Effect of methionine supplementation in chicken feed on the quality and shelf life of fresh poultry meat.

The aim of this study was to investigate the influence of different methionine sources and concentrations on the quality and spoilage process of broiler meat. The trial was comprised of 7 treatment groups: one basal group (suboptimal in Methionine+Cysteine; i.e.