Biomarkers of oxidative stress study V: ozone exposure of rats and its effect on lipids, proteins, and DNA in plasma and urine.

Ozone exposure effect on free radical-catalyzed oxidation products of lipids, proteins, and DNA in the plasma and urine of rats was studied as a continuation of the international Biomarker of Oxidative Stress Study (BOSS) sponsored by NIEHS/NIH. The goal was to identify a biomarker for ozone-induced oxidative stress and to assess whether inconsistent results often reported in the literature might be due to the limitations of the available methods for measuring the various types of oxidative products. The time- and dose-dependent effects of ozone exposure on rat plasma lipid hydroperoxides, malondialdehyde, F2-isoprostanes, protein carbonyls, methionine oxidation, and tyrosine- and phenylalanine oxidation products, as well as urinary malondialdehyde and F2-isoprostanes were investigated with various techniques.

Effect of FIO2 on oxidative stress during interval training at moderate altitude.

Purpose: To evaluate the effect of different fractions of inspired oxygen (FIO2) on oxidative stress during a high-intensity interval workout in trained endurance athletes residing at altitude.

Methods: Subjects (N = 19) were trained male cyclists who were residents of moderate altitude (1800-1900 m). Testing was conducted at 1860 m (PB 610-612 torr, PIO2 approximately 128 torr).

Lipid peroxidation in the skeletal muscle of hamsters with emphysema.

Current evidence suggests that skeletal muscle functional impairments present in emphysema and COPD patients may in part be a consequence of the disease condition per se. The mechanistic basis for these impairments is uncertain. Within the human population, it is difficult to control for confounding effects of concomitantly reduced activity levels.