Enhancing Doxorubicin Detection: Multiphase Electroextraction for Efficient and Affordable UHPLC-DAD Analysis in Saliva.

Attesting optimal drug concentrations in biological fluids is crucial to ensure precise dosage adjustment, to guarantee therapy adherence, and to manage side effects in chemotherapy. Accurate drug determination relies on liquid chromatography and advanced detectors, with sample preparation playing a pivotal role, especially in complex matrices such as biological fluids. This study introduces a multiphase electroextraction (MPEE) of doxorubicin (DOX) in saliva by utilizing a paper point, followed by ultra-high-performance liquid chromatography coupled to diode array detection.

Layer-by-layer assembly of PDDA/MWCNTs thin films as an efficient strategy for extraction of organic compounds from complex samples.

This article presents the assembly and characterization of poly(diallyldimethylammonium chloride)/multi-walled carbon nanotubes (PDDA/MWCNTs) thin films on borosilicate bottles using a layer-by-layer (LBL) approach. The thin films, consisting of 10 bilayers of coating materials, were thoroughly characterized using UV-VIS spectroscopy, scanning electron microscopy (SEM), and zeta potential measurements. The modified bottles were then utilized for the extraction of analytes with diverse acid-base characteristics, including drugs, illicit drugs, and pesticides, from saliva, urine, and surface water samples.

Efficient and easily scaled-up biosorbent based on natural and chemically modified macauba (Acrocomia aculeata) to remove Al, Mn and Fe from surface water contaminated with iron mining tailings.

The ruptures of tailings mine dams in the cities of Mariana and Brumadinho contaminated local Brazilian Rivers with toxic metals. Herein, we describe a scaled-up biosorbent based on natural macauba endocarp (NTE) and macauba endocarp chemically treated (TE) to remove Al, Mn and Fe from aqueous solutions. For the TE material: the variation of pH and temperature of water did not cause significant sorption interferences; the kinetics studies suggest a pseudo-second-order model; the adsorption isotherms revealed that the Langmuir equation was the best fit for Al and Mn, while the Freundlich equation best described the Fe; and the maximum adsorption capacities were between 0.

Multiphase electroextraction as a simple and fast sample preparation alternative for the digital image determination of doxorubicin in saliva.

Monitoring chemotherapeutic drugs in biological fluids is, in many cases, extremely important for dose adjustment, the maintenance of therapies, and the control of side effects. In this work, a method for determining the doxorubicin in saliva by digital image analysis (DIA) was optimised and validated. Images from a paper point were obtained using a conventional and cheap flatbed scanner at a 600 ppp resolution.

Blockade of interleukin seventeen (IL-17A) with secukinumab in hospitalized COVID-19 patients - the BISHOP study.

Background: Patients with severe COVID-19 seem to evolve with a compromised antiviral response and hyperinflammation. Neutrophils are critical players in COVID-19. IL-17A plays a major role in protection against extracellular pathogens and neutrophil attraction/activation.

Determination of rare earth elements in Fe-minerals using external calibration by LA-ICP-MS and application on Cauê Iron Formation (Brazil).

The rare earth elements (REE) composition in Fe-mineral phases is an important tool in iron formation studies to obtain information about parent rocks and environmental and paragenetic processes. However, the determination of REE presents some difficulties, such as the low concentration of these elements, matrix complexity and lack of iron matrix certified reference materials. The aim of the present work is to propose an analytical method to determine the REE plus Y (REE + Y) contents at trace levels in Fe-(hydr)oxides by the laser ablation ICP-quadrupoleMS technique, using external calibration.

Cellulose cone tip as a sorbent material for multiphase electrical field-assisted extraction of cocaine from saliva and determination by LC-MS/MS.

A porous and hydrophilic sorbent material was used in an extraction system, assisted by electric fields, for the extraction of cocaine in saliva and subsequent determination by ultra-high-performance liquid chromatography associated with sequential triple quadrupole mass spectrometry (UHPLC-MS/MS). The cellulose-based material was characterized by scanning electron microscopy, infrared spectroscopy, thermogravimetric analysis, and X-ray diffraction. The time and voltage variables applied in the extraction process were investigated through a Doehlert experimental design, and with the best conditions found (35min and 300 V) some validation parameters were evaluated.

Development and characterization of a 3D oral mucosa model as a tool for host-pathogen interactions.

The aim of this study was to (i) design, develop and validate a practical and physiologically relevant reconstituted in vitro oral mucosa tissue model and (ii) to assess its applicability in in vitro host-pathogen interactions with C. albicans and S. aureus.

Dengue in the State of Rio Grande do Norte, Brazil, 2010-2012.

Objective: To present results of virological surveillance and epidemiological aspects of dengue in the State of Rio Grande do Norte, Brazil.

Methods: A total of 1581 cases, reported from 2010 to 2012 at various health centres in the state, were analysed by viral isolation and/or RT-PCR for viral detection and typing. To identify whether different genotypes were circulating in the state during this period, sequencing of the complete E gene for DENV (1485 bp in length) was performed directly from patient serum samples.

Effect of Twice-Daily Blue Light Treatment on Matrix-Rich Biofilm Development.

Background: The use of blue light has been proposed as a direct means of affecting local bacterial infections, however the use of blue light without a photosensitizer to prevent the biofilm development has not yet been explored. The aim of this study was to determine how the twice-daily treatment with blue light affects the development and composition of a matrix-rich biofilm.

Methodology/principal Findings: Biofilms of Streptococcus mutans UA159 were formed on saliva-coated hydroxyapatite discs for 5 days.

Antibacterial effects of chemomechanical instrumentation and calcium hydroxide in primary teeth with pulp necrosis.

Purpose: This study's purposes were to: evaluate the antibacterial effect of chemomechanical instrumentation and a calcium hydroxide-based dressing in primary teeth with pulp necrosis secondary to trauma; and detect the presence of Fusobacterium nucleatum and black-pigmented rods in the canals of these teeth.

Methods: Microbiological sample collections (MSCs) were obtained: after coronal access to the canals of primary incisors (in 18 teeth; MSC 1); after chemomechanical instrumentation (in 10 teeth; MSC 2); and 72 hours after removal of intracanal medication (in 18 teeth; MSC 3). These samples were sent for microbiological processing.

Eighteen-month clinical and radiographic evaluation of two root canal-filling materials in primary teeth with pulp necrosis secondary to trauma.

Aim: This study compared clinically and radiographically the use of zinc oxide and eugenol cement (ZOE) and a commercial calcium hydroxide and polyethylene glycol-based paste (Calen(®) ) thickened with zinc oxide as root canal-filling materials for primary teeth with pulp necrosis secondary to trauma within 18months of follow up.

Material And Methods: Eligible patients of both genders aged 2years and 6months to 5years and 10months who had been referred for dental treatment at a pediatric dental trauma service and presented at least one anterior primary tooth (central and/or lateral incisor) with pulp necrosis secondary to traumatic injury were selected. Twenty-six children (n=31 teeth) with mean age of 3.

Gingival abscess due to an unusual nail-biting habit: a case report.

Aim: Nail-biting is one of the most frequent deleterious oral habits in children. It can result in systemic diseases or oral traumatic lesion. This report describes a case of gingival abscess in a child due to a fingernail-biting habit.

[Health and environmental indicator systems in health institutions].

This article presents a discussion on conceptual and methodological aspects involved in the establishment of a system of indicators for Health and Environment, with the purpose of integrating the management of research, education and health services institutes while also taking Biosafety into account. The initial task was the study of international indicator models, paying special attention to the World Health Organization model, more apropriate to this article, which was used in the process of collection, organization and synthesis of data. This work aims to create methodological instruments for the monitoring and evaluation of these procedures and support the decision making process.