Publications by authors named "Denise Santos Ruzene"

Biosurfactants are amphiphilic biomolecules with promising tensoative and emulsifying properties that find application in the most varied industrial sectors: environment, food, agriculture, petroleum, cosmetics, and hygiene. In the current work, a 2 full-factorial design was performed to evaluate the effect and interactions of pineapple peel and corncob as substrates for biosurfactant production by Bacillus subtilis LMA-ICF-PC 001. In a previous stage, an alkaline pretreatment was applied to corncob samples to extract the xylose-rich hydrolysate.

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Biosurfactants are eco-friendly compounds with unique properties and promising potential as sustainable alternatives to chemical surfactants. The current review explores the multifaceted nature of biosurfactant production and applications, highlighting key fermentative parameters and microorganisms able to convert carbon-containing sources into biosurfactants. A spotlight is given on biosurfactants' obstacles in the global market, focusing on production costs and the challenges of large-scale synthesis.

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The use of non-conventional carbon sources for biosurfactant-producing microorganisms is a promising alternative in fermentation to substitute costly substrates. So, the current research used pineapple peel as a cost-effective and renewable substrate because of its rich composition in minerals and sugars and high availability. Following a 2 full factorial design, a bacterial strain of Bacillus subtilis produced biosurfactants in fermentative media containing different concentrations of glucose and concentrated pineapple peel juice (CPPJ).

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Article Synopsis
  • * Research utilized a central composite design to analyze how various substrates, including glucose and pineapple peel, impact surface tension reduction and emulsification index.
  • * The results showed that pineapple peels can effectively replace some glucose and mineral salts, leading to a high surface tension reduction rate (57.744%) and maximum emulsification index (61.92%) under specific conditions.
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The reuse of agro-industrial waste has been a recurring issue since the 20th century. With a composition rich in carbohydrates and because of the massive amount of residue produced daily all over the world, corncob became a low-cost and suitable substrate to produce high added-value compounds. Biosurfactants are bioproducts of versatile applications due to their chemical structure with hydrophilic and hydrophobic regions.

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The biosurfactant production is characterized by high costs with substrates, which does not make them sufficiently competitive against synthetic surfactants. The insertion of alternative sources of low cost, especially agro-industrial residue, is an excellent alternative to make this competitiveness viable. An alkaline pretreatment was used to extract the hemicellulose from corncob in order to enhance its C5 fraction, common to vegetable biomasses.

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The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing.

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Fungi producing high xylanase levels have attracted considerable attention because of their potential industrial applications. Batch cultivations of Aspergillus terricola fungus were evaluated in stirred tank and airlift bioreactors, by using wheat bran particles suspended in the cultivation medium as substrate for xylanase and β-xylosidase production. In the stirred tank bioreactor, in physical conditions of 30°C, 300 rpm, and aeration of 1 vvm (1 l min⁻¹), with direct inoculation of fungal spores, 7,475 U l⁻¹ xylanase was obtained after 36 h of operation, remaining constant after 24 h.

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Synopsis of recent research by authors named "Denise Santos Ruzene"

  • - Denise Santos Ruzene's recent research focuses on the production of biosurfactants from agro-industrial waste, particularly using pineapple peel and corncob as cost-effective and sustainable substrates, demonstrating their potential for environmental and industrial applications!* - A series of experimental designs, including full-factorial and central composite designs, were employed to evaluate the optimal conditions for biosurfactant production, highlighting the significance of nutrient composition in enhancing microbial growth and biosurfactant yield.* - The findings underscore the economic viability and ecological benefits of utilizing agricultural waste materials, paving the way for innovative bioprocesses that address both waste management and the demand for renewable, eco-friendly surfactants.*