Efficient cartridge purification for producing high molar activity [F]fluoro-glycoconjugates via oxime formation.

Introduction: F-fluoroglycosylation via oxime formation is a chemoselective and mild radiolabeling method for sensitive molecules. Glycosylation can also improve the bioavailability, in vivo kinetics, and stability of the compound in blood, as well as accelerate clearance of biomolecules. A typical synthesis procedure for F-fluoroglycosylation with [F]FDG (2-deoxy-2-[F]fluoro-d-glucose) and [F]FDR (5-deoxy-5-[F]fluoro-d-ribose) involves two HPLC (high performance liquid chromatography) purifications: one after F-fluorination of the carbohydrate to remove its labeling precursor, and a second one after the oxime formation step to remove the aminooxy precursor.

Imaging and analysis of thin structures using positron emission tomography: Thin phantoms and in vivo tobacco leaves study.

In this work, a novel approach utilizing the designed phantoms imitating the plant tissues was applied for the evaluation of the relationships between the parameters of the prepared phantoms and/or quantitative variables obtained within the PET analysis. The microPET system developed for animal objects and approaches used made it possible to obtain the quantitative data in the form of (18)F radioactivity as well as the glucose (in µg) accumulated in leaf tissues within the dynamic in vivo study.