Publications by authors named "Denis V Goryunov"
Article Synopsis
- Rf1 is a crucial gene for restoring male fertility in sunflowers, but its exact location and function have yet to be fully determined.
- Researchers have developed two new markers based on single nucleotide polymorphisms (SNPs) linked to the Rf1 gene, which have proven effective for genotyping various sunflower accessions and populations.
- The study successfully pinpointed the Rf1 gene's location to a region of 59 Kb, narrowing down candidate genes but suggesting further analysis may uncover additional candidates not present in the reference genome of cytoplasmic male sterility maintainer lines.
Background: Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content in sunflower was under strong selection. Further improvement of oil properties achieved by modulating its fatty acid composition is one of the main directions in modern oilseed crop breeding.
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- The mitochondrial genome of moss was sequenced with Spades, containing 100,342 base pairs and a gene set similar to other bryophytes.
- The genome includes 66 genes, including rRNAs, tRNAs, and mitochondrial proteins, but notably lacks a functional gene found in most other bryophyte mitogenomes.
- The study suggests the independent evolution of certain genes and highlights the potential of the moss mitochondrial genome as a phylogenetic marker, supported by a phylogenetic tree based on 33 protein coding sequences from 40 bryophyte species.
PCR-based approach was used as a phylogenetic profiling tool to probe genomic DNA samples from representatives of evolutionary distant moss taxa, namely, classes Bryopsida, Tetraphidopsida, Polytrichopsida, Andreaeopsida, and Sphagnopsida. We found relatives of all Physcomitrella patens miR390 and TAS3-like loci in these plant taxa excluding Sphagnopsida. Importantly, cloning and sequencing of Marchantia polymorpha genomic DNA showed miR390 and TAS3-like sequences which were also found among genomic reads of M.
View Article and Find Full Text PDFThe aim of the study is a comparative investigation of changes that certain genome parts undergo during speciation. The research was focused on divergence of coding and noncoding sequences in different groups of salmonid fishes of the Salmonidae (Salmo, Parasalmo, Oncorhynchus, and Salvelinus genera) and the Coregonidae families under different levels of reproductive isolation. Two basic approaches were used: (1) PCR-RAPD with a 20-22 nt primer design with subsequent cloning and sequencing of the products and (2) a modified endonuclease restriction analysis.
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