Nanoparticles based on the zwitterionic pillar[5]arene and Ag: synthesis, self-assembly and cytotoxicity in the human lung cancer cell line A549.

For the first time, stable pillar[5]arene/Ag nanoparticles, consisting of water-soluble pillar[5]arene containing γ-sulfobetaine fragments and Ag ions without Ag-Ag bonds, were synthesized and characterized. The pillar[5]arene/Ag (ratio 1:10) nanoparticles obtained were cubic with a rib length of 100 nm and are less cytotoxic than Ag ions. The survival of the A549 model cells in the presence of pillar[5]arene/Ag (1:10) nanoparticles at a concentration of 30 and 40 μM was 76% and 55%, while in the absence of pillar[5]arene, the cell survival for free Ag ions at the same concentration was 30% and 10%, respectively.

Supramolecular neuromuscular blocker inhibition by a pillar[5]arene through aqueous inclusion of rocuronium bromide.

A water-soluble pillar[5]arene, decafunctionalized with thioether and carboxylate fragments, was synthesized as a structural analogue of Sugammadex. Its ability to restore the contraction of the diaphragm muscle by encapsulating the muscle relaxant rocuronium bromide was demonstrated. Using UV-vis, NMR and fluorescence spectroscopy, it was shown that the muscle relaxant is associated with the pillar[5]arene with an association constant of 4500 M-1 and a stoichiometry of 1 : 1.