W70: a probiotic with capacity to enhance ammonia assimilation in the rumen.

Ruminants excrete both undigested and excess consumed nitrogen (N) through feces and urine, which results in the waste of feed protein and environmental pollution. This study aims to screen bacteria to identify strains with potential to improve nitrogen utilization in the rumen. In a tube screening test, when 30 mmol/L ammonium sulfate was added as the only nitrogen source in liquid medium, among 115 bacterial strains belonging to and lactic-acid bacteria, 10 strains with the highest growth (OD level) in comparison to the other evaluated strains were identified, and of these, W70 and M50, had the highest ammonia nitrogen utilizing capabilities of ammonium sulfate (32.

Smallest microplastics intensify maize yield decline, soil processes and consequent global warming potential.

Microplastic pollution seriously affects global agroecosystems, strongly influencing soil processes and crop growth. Microplastics impact could be size-dependent, yet relevant field experiments are scarce. We conducted a field experiment in a soil-maize agroecosystem to assess interactions between microplastic types and sizes.

Proteomics and surface free fatty acid analysis of milk fat globules in spray- and freeze-dried bovine, goat, and horse milk powders.

Changes in the structure and composition of milk fat globules in spray- and freeze-dried milk powders have recently garnered notable attention. This study investigated changes in milk fat globular membrane (MFGM) proteins from bovine, goat, and horse milk powders, both spray- and freeze-dried, using a label-free proteomic approach, and quantified surface free fatty acids and their composition using GC. The results showed that several proteins, including α-CN and β-LG, increased, whereas fibrinogen α and β chain and mucin-1 decreased in the MFGM fractions of the studied spray-dried milk powders.

Application of isotope and multi-element fingerprinting in subregional milk authentication - The case of Ningxia, China.

Traceability of origin enhances the branding and safety of agricultural products. This study developed a subregional milk origin tracing method by selecting thirty large-scale farms: twenty-seven in Ningxia and three in Beijing. The farms were classified into six groups based on subregions: A (Yinchuan), B (Shizuishan), C (Qingtongxia), D (Yanchi), E (Zhongwei), and F (Beijing).

Dynamic changes in microbial communities and volatile compounds in kombucha fermentation using Flos sophorae and Elm fruits, compared to black and green tea.

The dynamic changes in physicochemical properties, microbial communities, and volatile compounds in kombucha made from Flos sophorae (FLSK) and Elm fruit (EFK) were compared to those of black tea (BTK) and green tea (GTK) over a 12-day fermentation period. The results revealed that overall flavonoid and polyphenol content, as well as antioxidant activity, increased initially and then decreased, accompanied by a steady reduction in pH within the fermentation broths investigated. Notably, the GTK exhibited stronger antioxidant activity than the other fermentation broths.

The effect of (Amla) fruit supplementation on the rumen microbiota and its correlation with rumen fermentation in dairy cows.

Introduction: Medicinal plants, rich in phytochemicals like phenolic acids, flavonoids, and tannins, offer potential benefits in enhancing productivity, quality, and animal health. Amla fruit () is one such plant with promising attributes. This study aimed to investigate the impact of fresh Amla fruit (FAF) supplementation on ruminal microbial composition and its correlation with rumen fermentation in lactating dairy cows.

Could soil microplastic pollution exacerbate climate change? A meta-analysis of greenhouse gas emissions and global warming potential.

Microplastics pollution and climate change are primarily investigated in isolation, despite their joint threat to the environment. Greenhouse gases (GHGs) are emitted during: the production of plastic and rubber, the use and degradation of plastic, and after contamination of environment. This is the first meta-analysis to assess underlying causal relationships and the influence of likely mediators.

Turning weeds into feed: Ensiling Calotropis gigantea (Giant milkweed) reduces its toxicity and enhances its palatability for dairy cows.

Calotropis gigantea (Giant milkweed, GM) has the potential to be utilized as a new feed additive for ruminants, however, the presence of unpalatable or toxic compounds decreases animal feed intake. This study aimed to valorize GM as a potential new feed resource through the chemical and microbial biotransformation of toxic compounds that will henceforth, make the plant palatable for cows. After GM's ensiling using fermentative bacteria, the plant was sampled for UHPLC-MS/MS to analyse the metabolomic changes.

Multi-omics dataset of bovine mammary epithelial cells stimulated by ten different essential amino acids.

Application of high-throughput sequencing and screening help to detect the transcriptional and metabolic discrepancies in organs provided with various levels of nutrients. The influences of individual essential amino acid (EAA) administration on transcriptomic and metabolomic profilings of bovine mammary epithelial cells (BMECs) were systematically investigated. A RNA sequencing and liquid chromatography-tandem mass spectrometry generated a comprehensive comparison of transcriptomics, non-targeted metabolomics and targeted amino acids profilings of BMECs with individual EAA stimulation by turn.

Schizochytrium sp. and lactoferrin supplementation alleviates Escherichia coli K99-induced diarrhea in preweaning dairy calves.

Calf diarrhea, a common disease mainly induced by Escherichia coli infection, is one of the main reasons for nonpredator losses. Hence, an effective nonantibacterial approach to prevent calf diarrhea has become an emerging requirement. This study evaluated the microalgae Schizochytrium sp.

The Effects of Prolonged Basic Amino Acid Exposures on Mitochondrial Enzyme Gene Expressions, Metabolic Profiling and Insulin Secretions and Syntheses in Rat INS-1 β-Cells.

In order to investigate the chronic effects of basic amino acids (BAA) on β-cell metabolism and insulin secretion, INS-1 β-cells were randomly assigned to cultures in standard medium (Con), standard medium plus 10 mM L-Arginine (Arg), standard medium plus 10 mM L-Histidine (His) or standard medium plus 10 mM L-Lysine (Lys) for 24 h. Results showed that insulin secretion was decreased by the Arg treatment but was increased by the His treatment relative to the Con group ( < 0.05).

Insight into differences in whey proteome from human and eight dairy animal species for formula humanization.

Human breastmilk fulfills the nutritional needs of infants and therefore is the best template for formula. In this study, whey proteins were investigated among human and eight dairy animal species using label-free proteomics approach. Totally, 965 proteins from milk whey were identified and large variations were observed between human and animals.

Pre-calving energy density and rumen protected lysine impacted blood metabolites and biomarkers of liver functions in dairy cows during the transition period.

Dairy cows usually face negative energy balance and disorders of normal organ function due to a mismatch between energy intake and energy demand. Negative energy balance directly affects liver function and blood metabolites because the liver is used as source of energy supply and a center of metabolic activity. This study was aimed to determine the effect of pre-calving energy density and rumen-protected lysine on blood metabolites and biomarkers of liver functions in the dairy cows during the transition period.

Corrigendum: Evolution, ecology, and zoonotic transmission of betacoronaviruses: a review.

[This corrects the article DOI: 10.3389/fvets.2021.

Sodium butyrate supplementation impacts the gastrointestinal bacteria of dairy calves before weaning.

The objective of this study was to systematically investigate how sodium butyrate (SB) affects the gastrointestinal bacteria in newborn calves at different stages before weaning. Forty female newborn Holstein calves (4-day-old, 40 ± 5 kg of body weight) were randomly divided into four groups; each group was supplemented with four SB doses: 0, 15, 30, and 45 g/day (ten replicates) in SB0, SB15, SB30, and SB45 groups, respectively. SB was fed with milk replacer from day 4 to day 60.

Corrigendum: Evolution, ecology, and zoonotic transmission of betacoronaviruses: A review.

[This corrects the article DOI: 10.3389/fvets.2021.

(Amla) Fruit Powder as a Supplement to Improve Preweaning Dairy Calves' Health: Effect on Antioxidant Capacity, Immune Response, and Gut Bacterial Diversity.

Disease is the main reason for the use of antimicrobials in calf rearing, and antibiotics are commonly used to treat calves, including for unknown diseases. This leads to antimicrobial resistance, which is a challenge to the livestock industry and public health. Plant products containing high levels of phytochemicals may improve the immunity and resistance of calves against infections, thereby reducing the use of antimicrobials.

Enhancing docosahexaenoic acid production of Schizochytrium sp. by optimizing fermentation using central composite design.

Docosahexaenoic acid (DHA) can improve human and animal health, particularly including anti-inflammatory, antioxidant, anticancer, neurological, and visual functions. Schizochytrium sp. is a marine heterotrophic protist producing oil with high DHA content, which is widely used in animal and food production.

MRCKα is a novel regulator of prolactin-induced lactogenesis in bovine mammary epithelial cells.

Myotonic dystrophy-related Cdc42-binding kinase alpha (MRCKα) is an integral component of signaling pathways controlling vital cellular processes, including cytoskeletal reorganization, cell proliferation and cell survival. In this study, we investigated the physiological role of MRCKα in milk protein and fat production in dairy cows, which requires a dynamic and strict organization of the cytoskeletal network in bovine mammary epithelial cells (BMEC). Within a selection of 9 Holstein cows, we found that both mRNA and protein expression of MRCKα in the mammary gland were upregulated during lactation and correlated positively ( > 0.

Feasibility of Supplying Ruminally Protected Lysine and Methionine to Periparturient Dairy Cows on the Efficiency of Subsequent Lactation.

The objective of this study was to evaluate the effects of supplying ruminally protected Lys (RPL) and ruminally protected Met (RPM) to transition cows' diets on the efficiency of subsequent lactation. A total of 120 prepartum Holstein cows were assigned into four treatments blocked by the anticipated calving date, previous lactation milk yield, number of lactations, and body condition score and fed either RPL, RPM, or the combination (RPML) or control diet (CON) throughout the transition period (3 weeks before till 3 weeks after calving). From 22 to 150 days in milk (DIM), all animals (100 cows) were fed a combination of RPM and RPL (0.

Heat stress impacts the multi-domain ruminal microbiota and some of the functional features independent of its effect on feed intake in lactating dairy cows.

Background: Heat stress (HS) affects the ruminal microbiota and decreases the lactation performance of dairy cows. Because HS decreases feed intake, the results of previous studies were confounded by the effect of HS on feed intake. This study examined the direct effect of HS on the ruminal microbiota using lactating Holstein cows that were pair-fed and housed in environmental chambers in a 2 × 2 crossover design.