[Analysis of polymorphism on ribosomal internal transcribed spacer 2 in four species of sandflies with PCR-RFLP].

Internal transcribed spacer 2 region in 4 species of sandflies from China (Phlebotomus chinensis, Ph. wui, Ph. longiductus and Ph.

[Investigation on transmitting vectors of visceral leishmaniasis in Jiashi County, Xinjiang].

Objective: To investigate sandfly vectors transmitting visceral leishmaniasis, including species and seasonal distribution in Jiashi county of Xinjiang Uygur Autonomous Region.

Methods: Sandflies were collected in the field, counted and identified. The specimens were dissected to analyze the gonotrophic cycle and to find infection of promastigotes.

[An epidemiological survey of visceral leishmaniasis in Hamangou coal mine area of Korla City, Xinjiang].

Objective: To investigate the epidemiological status of visceral leishmaniasis in Hamangou coal mine area of Korla City of Xinjiang Uygur Autonomous Region.

Methods: Based on a hint of possible existence of patients, a retrospective survey was carried out house by house to find cases with suspected signs/symptoms of the disease. Meanwhile, a survey on current status was conducted, including physical examination (liver and spleen palpation) to those less than 15 years-old, leishmanin skin test and rk39 immunochromatographic strip test for part of the residents.

[Field test of sandfly-catching by light traps and attractants].

Three kinds of light traps, attractants and their combination were used to collect sandflies in Andier township, Minfeng County of Xinjiang. The combined use of carbon dioxide and tungsten lamp showed better attraction effect to sandflies, also an easier way for the separation of insects collected.

[A newly identified endemic area of visceral leishmaniasis in Minfeng county of south Xinjiang II. Investigation on phlebotomine vectors].

Objective: To investigate the transmitting phlebotomine sandfly in Minfeng County, a newly-identified endemic area of visceral leishmaniasis in the south of Talim Pendi of Xinjiang.

Methods: Sandflies were collected using routine methods in and around the Yatonggusi village of Andier Township. The sandflies were identified to get their composition.

[A newly identified endemic area of visceral leishmaniasis in Minfeng county of South Xinjiang I. epidemiological survey].

Objective: To investigate the epidemiological status of visceral leishmaniasis in Minfeng county, a newly identified endemic area in south Xinjiang, China.

Methods: Based on a hint of possible existence of patients, a retrospective survey was carried out house by house in Andier Township of the county to find cases with suspected signs/symptoms of the disease in the past 20 years including those died. Meanwhile, a survey on current status was conducted, including physical examination(liver and spleen palpation) to those under 15 years-old, leishmanin skin test and rk39 immunochromatographic strip test for part of the residents.

[Preliminary test of phlebotomus surveillance by light-traps].

Different light-traps were tested in Wenxian of Gansu Province, including the light-traps without lamp and with glucose solution as attractant. Results showed that the light-traps attracted more mosquitoes and other insects than sandflies, and it became difficult to pick up the sandflies from gathering packet; the light-trap without lamp captured smaller amount of sandflies but much less other insects; glucose showed no significant effect in attracting sandflies. The existing light-traps are not so effective for Phlebotomus surveillance, and it is suggested that more effective attractants be tried.

JWA as a novel molecule involved in oxidative stress-associated signal pathway in myelogenous leukemia cells.

Previous data showed that JWA might be a novel environmental responsive gene regulated by environmental stressors such as heat shock and oxidative stress. However, the molecular mechanism underlying JWA gene function involved in oxidative stress is still unknown. In this study, the potential role of JWA was further investigated in hydrogen peroxide (H2O2) induced DNA damage and cell apoptosis in K562 cells.

[Expression deficiency of JWA enhanced DNA damage and delayed DNA repair in HeLa cells induced by benzo (a) pyrene exposure].

Objective: To investigate the role of JWA gene in benzo (a) pyrene [B (a) P] induced DNA damage and repair effects in HeLa cells.

Methods: The antisense JWA express vector (pEGFP-C1-asJWA) was constructed and stably transfected into HeLa cells. JWA deficient HeLa cells (asJWA-HeLa) was then screened and established.

[Expression of novel environmental responsive protein JWA involved in the oxidative stress responsiveness in MCF-7 cells].

Objective: To study the expression and the possible role of JWA protein in oxidative stress-induced damage of MCF-7 cells, especially the relationship between JWA and heat shock proteins (HSPs).

Methods: MCF-7 cells were exposed to different concentration of H(2)O(2) (0.01,0.

[Relationship between JWA expression and DNA damage-repair in human embryonic lung cells by benzo(a) pyrene].

Objective: To investigate the expressions of JWA gene and heat shock protein 70 (hsp70) in human embryonic lung (cccHPF-1) cells after exposure to activated benzo(a)pyrene (B(a)P), and to explore the role and the possible mechanism of JWA gene involved in B(a)P-induced DNA damage and repair.

Methods: The models of DNA damage of ccc-HPF-1 cells were established by treatment of cells with B(a)P plus S9 at 10 to 100 micromol/L for 3 hours with or without 24 hours recovery for DNA repairing. The DNA damage was detected by single cell gel electrophoresis (SCGE) assay (comet assay).