Comparative analysis of intestinal parasitic infections of outpatients in Guangxi medical university affiliated hospital in 2005 and 2013.

Objective: To understand the hospital's status and trends of intestinal parasitic infections and to provide a reference for prevention.

Methods: Stool samples were treated by acid-ether centrifugation; iodine staining and direct-smearing were performed; intestinal parasites were examined under a microscope; characteristics of parasitic infections in population were analyzed using the descriptive epidemiological method.

Results: 10 kinds of parasites were detected; the infection rate of clonorchissinensis was the highest, followed by B.

[PCR-based genotype classification of Blastocystis hominis isolates from college students of Guangxi].

Fifty-three Blastocystis hominis isolates were separated from the fecal specimens of carriers in college students from Guangxi and cultivated in vitro, and the genetic DNA was extracted. All the isolates were genotyped by PCR using seven pairs of known sequence-tagged site (STS) primers. The results showed there were five subtypes in the 53 isolates.

[Relationship between morphology and pathogenicity of Blastocystis hominis trophozoites].

Six hundred and eighty-six fresh fecal specimens were collected from outpatients (663 well-formed feces and 23 watery feces) during March 2011 to March 2012. All specimens were examined microscopically by direct smear and iodine stained method. B.

[Expressions and significance of IL-17 and IL-23 in intestinal mucosa of mice infected with Blastocystis hominis].

Objective: To study the expressions of interleukin-17 (IL-17) and interleukin-23 (IL-23) in the intestinal mucosa of BABL/C mice infected with Blastocystis hominis.

Methods: A total of 30 BABL/C mice were randomly divided into different groups: an experimental group, an immunosuppressant group and a normal group. Each mouse of the experimental group and immunosuppressant group was administered intraperieneally with dexamethasone (2 mg, gd, for 5 days) and one of the control group was given physiological saline (0.

[A preliminary study on preservation methods of Blastocystis hominis].

Objective: To observe the effect of different cryoprotective agents and temperature factors on the viability of Blastocystis hominis so as to explore the ideal method for preservation of B. hominis.

Methods: B.