Clinical trials using dental stem cells: 2022 update.

For nearly 20 years, dental stem cells (DSCs) have been successfully isolated from mature/immature teeth and surrounding tissue, including dental pulp of permanent teeth and exfoliated deciduous teeth, periodontal ligaments, dental follicles, and gingival and apical papilla. They have several properties (such as self-renewal, multidirectional differentiation, and immunomodulation) and exhibit enormous potential for clinical applications. To date, many clinical articles and clinical trials using DSCs have reported the treatment of pulpitis, periapical lesions, periodontitis, cleft lip and palate, acute ischemic stroke, and so on, and DSC-based therapies obtained satisfactory effects in most clinical trials.

SFRP2 enhances dental pulp stem cell-mediated dentin regeneration in rabbit jaw.

Objectives: Dental tissue-derived mesenchymal stem cell (MSC)-mediated tooth regeneration may be a useful therapeutic tool for repairing tooth loss. However, the low success rate of tooth regeneration restricts its clinical application. Identifying key factors for enhancing dentinogenesis in MSCs is crucial for promoting tooth regeneration.

Analysis of Coding RNA and LncRNA Expression Profile of Stem Cells from the Apical Papilla After Depletion of Sirtuin 7.

Objective: To explore the effects of Sirtuin 7 (SIRT7) on the gene expression profile of stem cells from the apical papilla (SCAPs).

Methods: SCAPs were isolated and cultured. SIRT7 short hairpin ribonucleic acid (shRNA) was used to knock down the expression of SIRT7 in SCAPs.

[Increase of saliva nitrate and nitrite level in patients with oral candidiasis].

Objective: To observe the alterations of saliva nitrate and nitrite level in patients with oral candidiasis.

Methods: Parotid saliva and whole saliva were collected from 33 patients and 34 healthy volunteers. Concentrations of nitrate and nitrite in saliva were determined by high-performance liquid chromatography.

[Alterations of salivary flow rate and oral main pathogens in miniature pig with bilateral parotid gland atrophy].

Objective: To observe the effect of bilateral parotid gland atrophy on the whole saliva flow rate and the growth of main oral pathogens in different sites of oral cavity.

Methods: Ten healthy miniature pigs were divided into two groups. The parotid glands of test group (n = 5) were bilaterally ablated by methyl violet.

Antimicrobial effect of acidified nitrate and nitrite on six common oral pathogens in vitro.

Background: Salivary nitrate is positively correlated with plasma nitrate and its level is 9 times the plasma level after nitrate loading. Nitrate in saliva is known to be reduced to nitrite by oral bacteria. Nitrate and nitrite levels in saliva are 3 - 5 times those in serum in physiological conditions respectively in our previous study.