Vitamin A acid (VAA) was firstly dissolved in chloroform, and then the organic solution containing the samples was mixed with silver sol at the desired concentration. After sufficient shaking, collect SERS spectra of VAA with silver sol layer solution. The attribution of the peaks was illustrated by comparing SERS spectra with NR (Normal Raman) spectra, and the mechanism and orientation of adsorption were also discussed according to the peaks which was absent in NR spectrum but observed in SERS spectrum.
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