Molecular cloning and characterization of caprine calpastatin gene.

Calpastatin (CAST) is an important gene for meat quality traits in livestock and poultry. The cDNA of caprine CAST gene was amplified for the first time using RACE-PCR. Results showed the full-length cDNA of caprine CAST gene (Accession no.

[Analysis of the fractal properties of the birth weight of Liangshan semi-fine wool sheep.].

Understanding of animal population genetic structure about quantitative traits is an important step in animal breeding. In the present study, the data of birth weight of the Liangshan semi-fine wool sheep was first studied by the fractal theory of nonlinear theory. The information dimension, correlation dimension, none-scale range, and the scale range were calculated.

[Differentiation of Bos grunniens, Bos Taurus, and Bubalus from meat products mixture based on mitochondrion 12S rRNA gene].

Mitochondrial DNA sequence is highly conserved within species. Gene 12S rRNA is able to endure degeneration and high temperature, which allows identification of feedstuff, fresh meat, processed meat, and traceability. In the present study, three unique restriction sites were detected in the fragments of mitochondrial 12S rRNA gene regions amplified with universal primer, which were able to distinguish Bos grunniens, Bos.

[Construction of genetic linkage map and QTL detection of chromosome 3 in sheep].

Based on the reference family of Sichuan liangshan semi-fine sheep, the genetic linkage map was constructed by Crimap 2.4 software, which was used to detect the QTLs for growth traits by QTLExpress with nine microsatellite markers located on chromosome 3 in sheep. The results indicated that: (1) the mean of the polymorphic information content was 0.

[SNPs of myostatin gene and its genetic effects on carcass traits in chicken].

Two polymorphic sites in the first exon 1 of MSTN gene in 180 Wenling grass-chicken including 3 lines were studied by PCR-RFLP method,and its genetic effects on carcass traits were researched. The results showed that 2 genotypes (MN and NN) in G(2100)A locus were identified by Bsh1236 I and 3 genotypes (AA, AB and BB) in G(2109)A locus were identified by Msp I, and 5 genotypes were emerged while com-analyzing 2 loci. The chi2 test indicated genotype frequencies had no significant deviation (P > 0.

[Correlation analysis of microsatellite DNA markers with wool traits in Liangshan semi-fine wool sheep].

Eighteen microsatellites on chromosomes 1, 2, 3, 9 were studied to detect their genotypes in 206 individuals in a Liangshan Semi-fine Wool Sheep nucleus breeding population. A GLM procedure was used to analyze the effects of these 18 microsatellites on wool traits. Results uncovered 7 loci that had a significant impact on wool traits.

[Study on sex-identification of ruminant using SRY gene and microsatellite markers].

Unlabelled: Multiplex PCR amplification of four microsatellite markers on chromosomes X and Y and the SRY gene was used to identify the sex and genotype the ruminant simultaneously in one step. Result indicated that the d SRY primers was highly specific and could be used as the main basis for sex-identification. But primer specificity was not good for Y chromosome markers MCM158 and MAF45, and therefore these markers were not suited for sex identification.

[A linkage map of mirosatellite on chromosome 1 in Liangshan semi-wool sheep].

The 9 microsatellite markers on Chromosomes 1 were used to construct a linkage map of Liangshan semi-wool sheep with a 387 members half-sib pedigrees after paternity testing. The results were as follows: the number of alleles for 9 markers was from 5 to 15; the heterozygosity was from 0.202 to 0.

[Microsatellite markers for paternity testing of Liangshan semi-fine wool sheep].

The usefulness of multiplex polymerase chain reaction was evaluated and fluorescent detection with 20 microsatellites was used for paternity testing of Liangshan semi-fine wool sheep. The results in Cervus 2.0 indicated that the combined exclusion probability was 0.