Publications by authors named "Dena Adachi"
Evidence is accumulating that quantitative hepatitis B surface antigen monitoring may be useful in managing patients with chronic HBV infection on certain treatment regimens. Based on these results with the Abbott Architect qualitative and quantitative HBsAg assays, it seems feasible to convert qualitative to quantitative HBsAg values for this purpose.
View Article and Find Full Text PDFA high-risk patient was informed of a positive HIV antibody/antigen test. However, follow-up samples taken 2-3 months later for HIV RNA and anti-HIV antibodies were negative. Human DNA testing confirmed that all samples were from this patient, excluding a sample mix-up.
View Article and Find Full Text PDFBackground: Hepatitis C (HCV) genotyping is important for treatment planning. The Abbott m2000 RealTime HCV Genotype II assay is a PCR-based assay targeting specific regions of the 5'NCR gene for genotypes 1-6, and the NS5b gene for subgenotypes 1a/1b. However, not all genotypes can be resolved, with results being reported as: 'indeterminate', 'mixed', 'genotype X reactivity with Y', or just the major genotype 1 alone.
View Article and Find Full Text PDFBackground: The rapidly evolving pandemic of novel 2009 swine-origin influenza A (H1N1) virus (S-OIV) demands that accurate and practical diagnostics be urgently evaluated for their potential clinical utility.
Objective: To determine the diagnostic accuracy of a rapid influenza diagnostic test (RIDT) and direct fluorescent antibody (DFA) assay for S-OIV by using reverse-transcription polymerase chain reaction (RT-PCR) as the reference standard.
Methods: We prospectively recruited children (aged 0-17 years) assessed in the emergency department of a pediatric referral hospital and a community pediatric clinic for influenza-like illness between May 22 and July 25, 2009.
We aimed to validate a direct immunofluorescence assay (DFA) for the detection of human metapneumovirus (hMPV) from nasal swabs and to determine the incidence and clinical features of this viral infection in a pediatric population. One hundred twenty-one of 3026 nasal swabs were positive for hMPV by DFA (4.0%).
View Article and Find Full Text PDFArticle Synopsis
- The study presents the first complete genomic sequence of human parainfluenza virus 4 (HPIV4), a virus whose genome was previously incomplete.
- The genomic sequence was obtained from a clinical isolate called SKPIV4 at a hospital in Toronto and shows high similarity (95%-97%) with existing partial sequences for HPIV4B.
- HPIV4 has the longest genome in the Rubulavirus genus at over 17 kb, providing valuable new information that will help further research on this respiratory virus.
Background: Severe acute respiratory syndrome (SARS) is a condition of unknown cause that has recently been recognized in patients in Asia, North America, and Europe. This report summarizes the initial epidemiologic findings, clinical description, and diagnostic findings that followed the identification of SARS in Canada.
Methods: SARS was first identified in Canada in early March 2003.