Publications by authors named "Den Sussland"
The group of Gram-positive bacillary organisms broadly known as "aerobic actinomycetes" consists of heterogeneous and taxonomically divergent genera. They are found in a wide variety of natural and man-made environments but are rarely considered a part of the normal human flora, with infections normally originating from exogenous sources. An extensive number of genera have been described, but only a minority of these has been associated with human or veterinary health.
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- The study focuses on improving the identification of Mycobacterium spp. and Nocardia spp. using MALDI-TOF MS, which is already popular in clinical labs but not yet optimal for these microbes.
- Researchers developed a database including over 2000 mass spectra from 494 strains, correcting ambiguous identifications using molecular analyses, leading to high accuracy in species identification.
- The identification success rate was 90% for Mycobacterium spp. and 88% for Nocardia spp., though some Mycobacterium isolates were difficult to identify due to protein masking; for well-represented species, accuracy was 95% or higher.
Recent reports of increasing in vitro sulfonamide resistance in Nocardia prompted us to investigate the findings. Despite the reports, there is a paucity of clinical reports of sulfonamide failure in treatment of nocardia disease. We reviewed 552 recent susceptibilities of clinical isolates of Nocardia from six major laboratories in the United States, and only 2% of the isolates were found to have resistant MICs of trimethoprim-sulfamethoxazole and/or sulfamethoxazole.
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