Post-Surgical Non-Invasive Wound Healing Monitoring in Oropharyngeal Mucosa.

Postoperative bleeding is the most significant complication of tonsillectomy. Regular monitoring of post-surgical wound healing in the pharynx is required. For this purpose, we propose endoscope-based non-invasive perfusion mapping and quantification.

Where in the tissues of Danio rerio is more H2O2 produced during acute hypoxia?

The lack of oxygen (O2) causes changes in the cell functioning. Modeling hypoxic conditions in vitro is challenging given that different cell types exhibit different sensitivities to tissue O2 levels. We present an effective in vivo platform for assessing various tissue and organ parameters in Danio rerio larvae under acute hypoxic conditions.

Influence of Compression Loading on Acoustic Emission and Light Polarization Features in TeO Crystal.

Monitoring the processes inside crystalline materials under their operating conditions is of great interest in optoelectronics and scientific instrumentation. Early defect detection ensures the proper functioning of multiple crystal-based devices. In this study, a combination of acoustic emission (AE) sensing and cross-polarization imaging is proposed for the fast characterization of the crystal's structure.

Microscopic photoplethysmography-based evaluation of cardiotoxicity in whitefish larvae induced by acute exposure to cadmium and phenol.

Toxic environmental pollutants pose a health risk for both humans and animals. Accumulation of industrial contaminants in freshwater fish may become a significant threat to biodiversity. Comprehensive monitoring of the impact of environmental stressors on fish functional systems is important and use of non-invasive tools that can detect the presence of these toxicants in vivo is desirable.

Optical transparency and label-free vessel imaging of zebrafish larvae in shortwave infrared range as a tool for prolonged studying of cardiovascular system development.

Optical techniques are utilized for the non-invasive analysis of the zebrafish cardiovascular system at early developmental stages. Being based mainly on conventional optical microscopy components and image sensors, the wavelength range of the collected and analyzed light is not out of the scope of 400-900 nm. In this paper, we compared the non-invasive optical approaches utilizing visible and near infrared range (VISNIR) 400-1000 and the shortwave infrared range (SWIR) 900-1700 nm.

Single-shot Mueller-matrix imaging of zebrafish tissues: In vivo analysis of developmental and pathological features.

Zebrafish is a well-established animal model for developmental and disease studies. Its optical transparency at early developmental stages allows in vivo tissues visualization. Interaction of polarized light with these tissues provides information on their structure and properties.

Exoscope-based videocapillaroscopy system for skin microcirculation imaging of various body areas.

The capillary system immediately responds to many pathologies and environmental conditions. Accurate monitoring of its functioning often enables early detection of various diseases related to disorders in skin microcirculation. To expand the scope of capillaroscopy application, it is reasonable to visualize and assess blood microcirculation exactly in the areas of inflamed skin.

Spectral Imaging Experiments with Various Optical Schemes Based on the Same AOTF.

Spectral image filtration by means of acousto-optical tunable filters (AOTFs) has multiple applications. For its implementation, a few different optical schemes are in use. They differ in image quality, number of coupling components, dimensions and alignment complexity.

Blood Vessel Imaging at Pre-Larval Stages of Zebrafish Embryonic Development.

The zebrafish () is an increasingly popular animal model biological system. In cardiovascular research, it has been used to model specific cardiac phenomena as well as to identify novel therapies for human cardiovascular disease. While the zebrafish cardiovascular system functioning is well examined at larval stages, the mechanisms by which vessel activity is initiated remain a subject of intense investigation.

Optimization of prism-based stereoscopic imaging systems at the optical design stage with respect to required 3D measurement accuracy.

We address the optical design procedure of prism-based stereoscopic imaging systems. Conventional approach includes two sequential stages: selection of the hardware and development of the proper digital image processing algorithms. At each of these stages, specific techniques are applied, which are almost unrelated to each other.

Imaging photoplethysmography and videocapillaroscopy enable noninvasive study of zebrafish cardiovascular system functioning.

We report on the noninvasive method for in vivo study of fish's cardiovascular system, that is, the heart and the structure of vessels that carry blood throughout the body. The proposed approach is based on combined photoplethysmographic and videocapillaroscopic microscopic imaging and enables noncontact two-dimensional mapping of blood volume changes. We demonstrate that the obtained data allows precise measurements of heartbeat, blood flow velocity and other important parameters (see Videos S1 and S2).

Modeling and optimization of a geometrical calibration procedure for stereoscopic video endoscopes.

Stereoscopic video endoscopes are widely used for remote visual inspection and precise three-dimensional (3D) measurements in industrial and biomedical applications. The reconstruction of 3D points from the corresponding image points requires a geometrical calibration procedure, the accuracy of which affects the measurement uncertainty. We propose to perform an optimal choice of the calibration technique and the calibration target parameters using a computer simulation at the design stage.

Imaging system based on a tandem acousto-optical tunable filter for in situ measurements of the high temperature distribution.

We present, to the best of our knowledge, the first experimental demonstration of a new imaging system for in situ measurement of the two-dimensional (2D) distribution of the surface temperature of microscopic specimens. The main component of the system is an imaging tandem acousto-optical tunable filter (TAOTF) synchronized with a video camera. A set of TAOTF spectroscopic images (up to a few hundreds) is taken by the TAOTF imaging system to fit the measured spectral curves in each pixel to the Planck radiation function and determine the temperature and emissivity of the sample using the gray body approximation.