Publications by authors named "Demetrio Alonso Ambriz-Garcia"
Article Synopsis
- Somatic cell nuclear transfer (SCNT) shows promise for cloning valuable animals and aiding conservation, but its success rate is hindered by developmental issues and epigenetic changes in cloned embryos.
- A study assessed the impact of Ovum Fluid-Umbilical Fluid (OF-UF) on intracellular levels of reactive oxygen species (ROS) and glutathione (GSH) in cloned and parthenogenetic embryos.
- Results indicated that 0.5% OF-UF had no positive effects, while 1% OF-UF negatively affected cloned embryos at the blastocyst stage, and parthenogenetic embryos experienced developmental issues at 2% OF-UF concentration, with no improvements seen in overall embryonic
The in vitro production and cryopreservation of mammalian embryos generates reactive oxygen species (ROS) due to conditions of the system that can overcome their antioxidant protection. Resveratrol is an antioxidant used in in vitro systems to improve blastocyst rates, but its effect on antioxidant enzymes such as glutathione (GSH) in embryos produced by in vitro fertilization (IVF) after vitrification has not been reported. The objective of this study was to evaluate the effects of resveratrol on the in vitro maturation medium (IVM) of sheep oocytes () on the levels of ROS and GSH in embryos produced by IVF subjected to vitrification.
View Article and Find Full Text PDFSomatic cell nuclear transfer (SCNT) is a reproductive biotechnology with great potential in the reproduction of different species of zootechnical interest, including sheep. This study aimed to carry out a bibliometric analysis of scientific papers published on the application of SCNT in sheep reproduction during the period 1997-2023. The search involved the Science Citation Index Expanded and Social Sciences Citation Index databases of the main collection of the Web of Sciences with different descriptors.
View Article and Find Full Text PDFBackground: The collection of ovaries from slaughterhouses is an important source of oocytes for embryo production. On the other hand, the physiological stage of slaughtered females varies and influences embryo production.
Objectives: The study examined the efficiency of embryos and demi-embryos from young, non-pregnant adult, and pregnant adult ewes from a local slaughterhouse.
The developmental dynamics of cloned Mexican bighorn sheep (Ovis canadensis mexicana) embryos were evaluated based on morphological quality standards. Categories determined by standards were correlated with the embryonic development stage, number of nuclei and viability. The results showed no differences in the blastocyst rate between the experimental (cloned Mexican bighorn sheep embryos) and control (parthenogenetic domestic sheep embryos) groups (p > .
View Article and Find Full Text PDF