Phage probe on RAFT polymer surface for rapid enumeration of E. coli K12.

This study presents a simple, fast, and sensitive label-free sensing assay for the precise enumeration of modeled pathogenic Escherichia coli K12 (E. coli K12) bacteria for the first time. The method employs the covalent binding bacteriophage technique on the surface of a reversible addition-fragmentation chain transfer (RAFT) polymer film.

Polyphasic characterization and genomic insights into Nocardioides turkmenicus sp. nov. isolated from a desert soil.

Strain KC13, a novel desert-adapted, non-motile, Gram-stain-positive, rod-shaped, aerobic bacterium, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan and characterised by a polyphasic approach. Phylogenetic analysis based on 16S rRNA sequences revealed that strain KC13 was a member of the genus Nocardioides, and formed a distinct cluster with Nocardioides luteus DSM 43366 (99.3% sequence identity), Nocardioides albus DSM 43109 (98.

In vitro genotoxic and antigenotoxic effects of an exopolysaccharide isolated from Lactobacillus salivarius KC27L.

Exopolysaccharide isolated from Lactobacillus salivarius (new genus name Ligilactobacillus) KC27L strain (EPS) exhibits antioxidant properties with 1,1-diphenyl-2-picrylhydrazase (DPPH) radical and superoxide anion radical (O) scavenging effect and iron ion (Fe) chelating activity. This study aimed to investigate the in vitro genotoxic effects of EPS alone (12.50, 25.

Enumeration in a Capillary-Driven Microfluidic Chip with SERS.

Pathogen detection is still a challenging issue for public health, especially in food products. A selective preconcentration step is also necessary if the target pathogen concentration is very low or if the sample volume is limited in the analysis. Plate counting (24-48 h) methods should be replaced by novel biosensor systems as an alternative reliable pathogen detection technique.

Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220, was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora. 16S rRNA gene sequence analysis of strain K220 showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12, Saccharopolyspora kobensis JCM 9109 and Saccharopolyspora hirsuta ATCC 27875 with 97.

Influence of graphene oxide on the toxicity of polystyrene nanoplastics to the marine microalgae Picochlorum sp.

Graphene oxide (GO) features distinctive physical and chemical characteristics; therefore, it has been intensively investigated in environmental remediation as a promising material for clean-up of soil contamination and water purification and used as immobilization material. Plastic is a widespread pollutant, and its breakdown products such as nanoplastics (NPs) should be evaluated for potential harmful effects. This study is aimed to evaluate the influence of GO on the toxicity of polystyrene (PS) NPs to the marine microalgae Picochlorum sp.

sp. nov., isolated from the top soil layer on basaltic material in Turkey.

An actinobacterium, designated 14C53, was isolated from a soil sample on basaltic material from Samsun, Turkey. The growth ranges for NaCl concentration and pH of strain 14C53 were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53 was most closely related to A8036 (98.

Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis.

Exploration of unexplored habitats for novel actinobacteria with high bioactivity potential holds great promise in the search for novel entities. During the course of isolation of actinobacteria from desert soils, four actinobacteria, designated as 5K548, 7K502, 16K309 and 16K404, were isolated from the Karakum Desert and their bioactivity potential as well as taxonomic provenances were revealed by comprehensive genome analyses. Pairwise sequence analyses of the 16S rRNA genes indicated that the four strains are representatives of putatively novel taxa within the prolific actinobacterial genus Saccharopolyspora.

Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil.

During a study to isolate such actinobacteria with unique metabolic potential, a novel actinobacterium, designated KC333, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of the strain was investigated using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was most closely related to Nonomuraea terrae CH32 (99.

Multiplex enumeration of Escherichia coli and Salmonella enteritidis in a passive capillary microfluidic chip.

Multiplex detection and quantification of bacteria in water by using portable devices are particularly essential in low and middle-income countries where access to clean drinking water is limited. Addressing this crucial problem, we report a highly sensitive immunoassay sensor system utilizing the fluorescence technique with magnetic nanoparticles (MNPs) to separate target bacteria and two different types of quantum dots (CdTe and Ni doped CdTe QDs) incorporated into a passive microfluidic chip to transport and to form sandwich complexes for the detection of two target bacteria, namely Escherichia coli (E. coli) and Salmonella enteritidis (S.

Streptomyces boluensis sp. nov., isolated from lake sediment.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537 showed that it is closely related to the type strain of Streptomyces ziwulingensis F22 (97.

Investigation of the toxic effects of different polystyrene micro-and nanoplastics on microalgae Chlorella vulgaris by analysis of cell viability, pigment content, oxidative stress and ultrastructural changes.

Plastics of different sizes (micro- and nano-sized) are often identified in aquatic environments. Nevertheless, their influence on marine organisms has not been widely investigated. In this study, the responses of the microalga Chlorella vulgaris to micro- and nanoplastics exposure were examined using long term toxicity test.

Nonomuraea basaltis sp. nov., a siderophore-producing actinobacteria isolated from surface soil of basaltic parent material.

A Gram-stain-positive, aerobic, spore-forming actinobacterial strain, designated 160415, was isolated from a surface soil sample, which was formed on basaltic parent material, collected from Samsun, Turkey. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 160415 clustered closely with species of the genus Nonomuraea, and showed the highest sequence similarity to Nonomuraea zeae NEAU-ND5, Nonomuraea candida HMC10 and Nonomuraea turkmeniaca DSM 43926 with 99.1%, 98.

sp. nov., isolated from desert soil and reclassification of as a later heterotypic synonym of .

A novel actinobacterial strain, designated 13K301, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of strain 13K301 was revealed by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain 13K301 belongs to the genus and had highest sequence similarity to '' S10 (99.

Genome-based classification of Micromonospora craterilacus sp. nov., a novel actinobacterium isolated from Nemrut Lake.

A novel actinobacterial strain, designated NA12, was isolated from coastal sediment sample of Nemrut Lake, a crater lake in eastern Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Cultural and chemotaxonomic characteristics of the strain were consistent with its classification within the family Micromonosporaceae.

Polyphasic classification of strains isolated from the Karakum Desert and description of sp. nov., sp. nov., sp. nov. and sp. nov.

Five actinobacteria isolates, KC201, KC401, KC310, KC712 and 6K102, were recovered from the Karakum Desert during an investigation of novel actinobacteria with biotechnological potential. A polyphasic approach confirmed the affiliation of the strains to the genus . The strains showed chemotaxonomic and morphological properties consistent with their classification in the genus .

Micromonospora orduensis sp. nov., isolated from deep marine sediment.

A novel actinobacterial strain, designated S2509, was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose.

sp. nov., isolated from the Karakum Desert.

An isolate, 13K206, with typical morphological characteristics of the genus was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliation of the strain. The strain showed chemotaxonomical properties consistent with its classification in the genus such as - and 3-OH-Apm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.

Fast fluorometric enumeration of E. coli using passive chip.

In this report, a passive microfluidic chip design was developed for fast and sensitive fluorometric determination of Escherichia coli (E. coli) based on sandwich immunoassay. Initially, magnetic nanoparticles (MNPs) and chitosan modified mercaptopropionic acid capped cadmium telluride (CdTe) quantum dots (QDs) were functionalized with E.

The coupling of immunomagnetic enrichment of bacteria with paper-based platform.

In this study, the coupling of magnetic enrichment of bacteria from real samples with rapid surface enhanced Raman spectroscopy (SERS) detection was reported. The selective isolation and enrichment for the model bacteria Escherichia coli (E. coli) was performed using E.

SERS-based rapid assay for sensitive detection of Group A Streptococcus by evaluation of the swab sampling technique.

Beta-hemolytic, Group A Streptococcus pyogenes (GAS) is a life-threating pathogen and the reason for prominent disease, pharyngitis. The conventional analysis of GAS, gold standard, takes 48 hours and the related rapid tests lack in accuracy and sensitivity. In this study, firstly, the efficiency of swab sampling, which is a must in the GAS detection, was discussed with the proposed surface-enhanced Raman spectroscopy (SERS)-based batch assay and each step was controlled by the plate-counting method.

Tracing Size and Surface Chemistry-Dependent Endosomal Uptake of Gold Nanoparticles Using Surface-Enhanced Raman Scattering.

Surface-enhanced Raman scattering (SERS)-based single-cell analysis is an emerging approach to obtain molecular level information from molecular dynamics in a living cell. In this study, endosomal biochemical dynamics was investigated based on size and surface chemistry-dependent uptake of gold nanoparticles (AuNPs) on single cells over time using SERS. MDA-MB-231 breast cancer cells were exposed to 13 and 50 nm AuNPs and their polyadenine oligonucleotide-modified forms by controlling the order and combination of AuNPs.

Jiangella anatolica sp. nov. isolated from coastal lake soil.

A novel actinobacterial strain, designated GTF31, was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835 (99.

Surface-enhanced Raman spectroscopy based 3D spheroid culture for drug discovery studies.

Three-dimensional (3D) spheroid cultures are more realistic tissue mimicking structures for drug discovery studies. However, analysis of 3D spheroid cultures is a challenge task because available techniques are destructive, which results with the loss of biochemical information confined in a spatial arrangement inside of spheroids. In this study, a surface-enhanced Raman scattering (SERS) based non-destructive approach is reported to study 3D cultures.