A New Validated Method for Rapid Determination of OLEU Concentration in Dietary Supplements: Comparison with Total Phenol Content and Antioxidant Activity.

The market for olive leaf dietary supplements is expanding rapidly and is valued at $437.15 million today. However, information on the control of these products is sketchy and the origin and variety of olives are rarely stated.

Influence of Oleacein, an Olive Oil and Olive Mill Wastewater Phenolic Compound, on Longevity and Stress Resistance.

Oleacein, a bioactive compound of olive oil and olive mill wastewater, has one of the strongest antioxidant activities among olive phenolics. However, few reports explore the in vivo antioxidant activity of oleacein, with no clear identification of the biological pathway involved. Earlier studies have demonstrated a link between stress resistance, such as oxidative stress, and longevity.

Development and cross-validation of simple HPLC-fluorescence and UPLC-MS-UV methods for rapid determination of oleuropein in olive leaves.

Introduction: Olive leaves, abundant by-products of the olive oil industry, are a rich source of oleuropein, an important polyphenol in olive leaves. So far, no published methods have been validated using matrix standards for oleuropein quantification in olive leaves.

Objectives: The study aimed to develop an HPLC method for oleuropein determination in olive leaves using spiked matrix standards prepared from a blank olive leaf matrix, to validate the method with respect to aqueous standards, and cross-validate the HPLC method with UPLC-MS and UPLC-UV techniques.