Effect of TetR Family Transcriptional Regulator PccD on Phytosterol Metabolism of .

Androstenedione (AD) is an important intermediate for the production of steroidal drugs. The process of transforming phytosterols into AD by is mainly the degradation process of the phytosterol side chain, and the excessive accumulation of propionyl-CoA produced by will produce toxic effects, which seriously restricts the transformation performance of strains. In this study, sp.

A novel lytic polysaccharide monooxygenase from enrichment microbiota and its application for shrimp shell powder biodegradation.

Lytic polysaccharide monooxygenases (LPMO) are expected to change the current status of chitin resource utilization. This study reports that targeted enrichment of the microbiota was performed with chitin by the selective gradient culture technique, and a novel LPMO (M2822) was identified from the enrichment microbiota metagenome. First, soil samples were screened based on soil bacterial species and chitinase biodiversity.

New Insights into the Modification of the Non-Core Metabolic Pathway of Steroids in and the Application of Fermentation Biotechnology in C-19 Steroid Production.

Androsta-4-ene-3,17-dione (AD), androsta-1,4-diene-3,17-dione (ADD), and 9α-hydroxy-4-androstene-3,17-dione (9-OHAD), which belong to C-19 steroids, are critical steroid-based drug intermediates. The biotransformation of phytosterols into C-19 steroids by cell factories is the core step in the synthesis of steroid-based drugs. The production performance of engineered mycolicibacterial strains has been effectively enhanced by sterol core metabolic modification.

Heavy metals passivation driven by the interaction of organic fractions and functional bacteria during biochar/montmorillonite-amended composting.

The aim of this study was to identify critical driving factors and pathways of mitigating heavy metals (HM) bioavailability during biochar/montmorillonite-amended composting: emphasize on the interaction effect between organic constituents and functional bacteria. Organic components, such as humus (HS), humic (HA) and fulvic acid (FA) and dissolved organic carbon (DOC), exhibited indivisible links with Cu and Zn speciation, which confirmed their vital roles on deactivating Cu and Zn. Network analysis indicated that biochar/montmorillonite obviously increased the diversity of Cu resistant/actor and Zn actor bacteria, which aided in HM passivation.