Phosphorus NMR analysis of human white matter in mixed non-ionic detergent micelles.

In order to study the lipid composition of human white matter, we have developed a 31P NMR spectroscopy method, which allows the determination and quantitation of the main phospholipids found in biological membranes. The technique is based upon the use of a non-ionic detergent (Triton X-100) which induces, in aqueous media, the formation of mixed micelles that are magnetically isotropic. The linewidths and chemical shifts depend on both the molar ratio detergent/phospholipid and the pH of the suspension.

[Perspectives in the use of NMR for the biochemical study of the white matter of the brain].

This paper presents a survey of the function and the biochemical composition of the myelin sheath. Proton nuclear magnetic resonance spectroscopy of extracts of human white matter tissue is a major method for the identification of myelin constituants. We present results of some experiments obtained with normal brain material at 80 MHz and 400 MHz.

Estimating meconium (fetal feces) concentration in human amniotic fluid by nuclear magnetic resonance.

Measurements are reported of NMR relaxation times (T2) on hydrogen in water of isotonic NaCl solutions and of normal human amniotic fluid in which known concentrations of meconium (fetal feces) were dissolved. Magnetic fields for resonance of 23.5 X 10(3) and 0.