Expression of pheromone receptor gene families during olfactory development in the mouse: expression of a V1 receptor in the main olfactory epithelium.

In the mouse, two large gene families, V1R and V2R, encoding putative pheromone receptors have been described. Studies have suggested a homotypic recognition role for V1Rs and V2Rs during development in the targeting of vomeronasal axons to specific sets of glomeruli in the accessory olfactory bulb (AOB). Analysis of the onset of expression of the V1R and V2R gene families in developing vomeronasal neurons using polymerase chain reaction and in situ hybridization now suggests that a role for these receptors in the organization of axon projections is only likely at the final stages of targeting within the AOB.

Disruption of ephrin signaling associates with disordered axophilic migration of the gonadotropin-releasing hormone neurons.

Ephrin signaling is involved in repulsive and attractive interactions mediating axon guidance and cell-boundary formation in the developing nervous system. As a result of a fortuitous transgene integration event, we have identified here a potential role for EphA5 in the axophilic migration of gonadotropin-releasing hormone (GnRH) neurons from the nasal placode into the brain along ephrin-expressing vomeronasal axons. Transgene integration in the GNR23 mouse line resulted in a 26 kb deletion in chromosome 5, approximately 67 kb 3' to Epha5.