Nondestructive Detection of Egg Freshness Based on Infrared Thermal Imaging.

In this paper, we proposed a nondestructive detection method for egg freshness based on infrared thermal imaging technology. We studied the relationship between egg thermal infrared images (different shell colors and cleanliness levels) and egg freshness under heating conditions. Firstly, we established a finite element model of egg heat conduction to study the optimal heat excitation temperature and time.

Inhibitory of active dual cancer targeting 5-Fluorouracil nanoparticles on liver cancer and .

The chitosan (CS) material as the skeleton nano-drug delivery system has the advantages of sustained release, biodegradability, and modifiability, and has broad application prospects. In the previous experiments, biotin (Bio) was grafted onto CS to synthesize biotin-modified chitosan (Bio-CS), and it was confirmed that it has liver cancer targeting properties. Single-targeted nanomaterials are susceptible to pathological and physiological factors, resulting in a state of ineffective binding between ligands and receptors, so there is still room for improvement in the targeting of liver cancer.

Origin of proton induced fluorescence quenching of colloidal carbon dots: reshaping of Schrödinger wavefunctions and huge red shift of transition energy.

The fluorescence quenching by protons is a universal phenomenon but the mechanism remains unclear. Here, we take the fluorescent amide-terminated carbon dots as a prototype to study the proton fluorescence quenching mechanism by using both experiments and time-dependent density functional theory calculations. The study reveals that when an approached proton is captured by the weakly negatively charged fluorophore group of the colloidal carbon dot, it will substantially change the electron wavefunctions owing to the strong proton-electron interaction, and this leads to highly diminished energy gap and resultant fluorescence quenching in the visible spectral region.

Nondestructive Detection for Egg Freshness Based on Hyperspectral Scattering Image Combined with Ensemble Learning.

Scattering hyperspectral technology is a nondestructive testing method with many advantages. Here, we propose a method to improve the accuracy of egg freshness, research the influence of incident angles of light source on the accuracy, and explain its mechanism. A variety of weak classifiers classify eggs based on the spectra after preprocessing and feature wavelength extraction to obtain three classifiers with the highest accuracy.

Anti-cancer efficacy of biotinylated chitosan nanoparticles in liver cancer.

The present study investigated the synthesis of biotinylated chitosan (Bio-CS) from chitosan using a nanomaterial skeleton with biotin and the successful targeting of the formulation in liver cancer cells. Bio-CS was validated by fourier transformed infrared spectroscopy and hydrogen nuclear magnetic resonance spectroscopy. Bio-CS and plasmid DNA were used to construct Bio-CS/plasmid DNA nanoparticles according to the optimal molar ratio of 1:1 and the optimal pH-value of 5.

Influence of physical and biological variability and solution methods in fruit and vegetable quality nondestructive inspection by using imaging and near-infrared spectroscopy techniques: A review.

Over the past decades, imaging and spectroscopy techniques have been rapidly developing and widely applied in nondestructive fruit and vegetable quality assessment. The physical properties (including size, shape, color, position, and temperature) and biological properties (including cultivar, season, maturity level and geographical origin) of fruits and vegetables vary from one to another. A great variety of physical and biological properties of agricultural products influence the optical propagation properties and interaction behaviors with incident light, thus decreasing the quality inspection accuracy.

Giant photoluminescence enhancement in SiC nanocrystals by resonant semiconductor exciton-metal surface plasmon coupling.

We report giant fluorescence enhancement in SiC nanocrystals (NCs) embedded in a sodium dodecyl sulfonate dielectric medium by proximately contacted Ag nanoparticles. The enhancement in integrated fluorescence intensity reaches an astonishing 176-fold under 360 nm excitation (53.3-fold enhancement in emission maximum intensity).

Highly bright tunable blue-violet photoluminescence in SiC nanocrystal-sodium dodecyl sulfonate crosslinked network.

We report strong photoluminescence in an ultra-small surface oxidized SiC quantum dot-sodium dodecyl sulfonate crosslinked network. The peak emission wavelength is tunable spanning a wide blue-violet spectral region showing clear quantum confinement effects. The photoluminescence decay exhibits triple recombination dynamics with an average lifetime of 13.

Survivin deficiency induces apoptosis and cell cycle arrest in HepG2 hepatocellular carcinoma cells.

The postulated dual roles of survivin as an anti-apoptotic factor and a mitotic inducer have placed this factor in the spotlight of cancer research. The purpose of this study was to investigate whether survivin might connect the cell cycle with apoptosis. Here, by simultaneously monitoring survivin deficiency-induced morphological changes of HepG2 cells using time-lapse imaging as well as determining apoptosis progression, we observed synchronized defective mitosis characterized by multinucleated and polyploid cells and cell cycle arrest at S phase or G2/M phase followed by apoptosis, the processes of which depended on the simultaneous destruction of specialized subcellular compartments of survivin and activation of caspase-3-like protease.

Effect of lentivirus-mediated shRNA targeting VEGFR-3 on proliferation, apoptosis and invasion of gastric cancer cells.

It has been reported that vascular endothelial growth factor receptor 3 (VEGFR-3) is highly expressed in most tumor tissues, including gastric cancer. However, the effects of VEGFR-3 knockdown on the proliferation, apoptosis and invasion of gastric cancer cells and downstream signaling molecules have not yet been well established. In the present study, four short hairpin RNA (shRNA) sequences targeting the VEGFR-3 gene (NM_002020) were designed and cloned into a lentiviral vector, pRNAT-U6.

Chemoresponse to docetaxel correlates with expression of the survivin splicing variants in patients with gastric cancer.

Background/aims: Survivin, a potential predictive marker to chemotherapeutic drugs, reduces the susceptibility of tumor cells to proapoptotic stimuli, thereby promoting tumor cell survival during tumor treatment with anticancer agents. In the present study, we examined the correlation between drug-response and expression of survivin in gastric cancer.

Methodology: Drug-response was performed by histoculture drug-response assay (HDRA) in 42 patients with advanced gastric cancer.

[Therapeutic effect of liver transplantation on acute liver failure].

Objective: To evaluate the short-term and long-term outcomes of emergent liver transplantation recipients with acute liver failure and to identify factors that influenced these outcomes.

Methods: 318 consecutive patients who underwent liver transplantations between January 2001 and December 2004 were analyzed retrospectively (all the cases were followed up to December 2005). According to UNOS grading scale, all recipients preoperative status were evaluated.

[The effect of antisense survivin-liposome complex on cell growth, apoptosis and cell cycle in hepatocellular carcinoma cells].

Objective: To investigate the effects and the mechanisms of cell growth inhibition in hepatocellular carcinoma cells after induction with antisense survivin-liposome (LIP) complex, and to provide evidence in treatment for hepatocellular carcinoma and tumors expressing survivin.

Methods: Survivin ODNs was transfected into HepG2 cells mediated by LiP reagent. The expression of survivin mRNA and protein was detected by RT-PCR and Western blot.

[Prognostic significance of survivin splicing variants in expression gastric cancer].

Objective: To study the expression and associations of three survivin splicing variants in gastric cancer and normal gastric mucosa, and to evaluate the prognostic significance.

Methods: Real time quantitative RT-PCR was used to detect the expression of three survivin splicing variants in tumor and matched normal gastric mucosa specimens from 77 cases with gastric cancer.

Results: The expression of three survivin splicing variants than upregulated significantly in gastric cancer than those in normal mucosas (P< 0.

[Effect of inhibiting survivin expression with antisense oligodeoxynucleotides on sensitivity of hepatocellular carcinoma cell lines HepG2 and HepG2/ADM to adriamycin].

Background & Objective: Survivin deserves attention as a selective target for cancer therapy because it is silenced in differentiated adult tissues, but is expressed in a variety of human tumors, and is involved in tumorigenesis and chemoresistance. Antisense oligodeoxynucleotides (ASODN) can be used to inhibit the expression of survivin to induce apoptosis or enhance chemosensitivity of tumor cells. This study was to investigate the effect of inhibiting survivin expression with ASODN on sensitivity of hepatocellular carcinoma cell lines HepG2 and HepG2/ADM to Adriamycin (ADM).

[Elevated expression level of wild-type survivin in gastric cancer promotes in vitro docetaxel-resistance].

Objective: To study the expression of three survivin splicing variants in gastric cancer and to evaluate the significant correlation between survivin variants' expression and chemoresistance in gastric cancer.

Methods: Real time quantitative RT-PCR was used to analyze the mRNA expression of survivin variants in 39 gastric tumor specimens resected during operation. The clinical resistance to anticancer agents [CDDP, MMC, 5-Fu, docetaxel (Taxotere TXT), and GEM] was analyzed by histoculture drug-response assay (HDRA).

Survivin antisense compound inhibits proliferation and promotes apoptosis in liver cancer cells.

Aim: To evaluate the effects of survivin on cell proliferation and apoptosis in liver cancer.

Methods: MTT assay was used to generate and optimize phosphorothioate antisense oligonucleotides (ODNs)-Lipofectamine2000 (LiP) compound by varying ODNs (mug):LiP (muL) ratios from 1:0.5 to 1:5.

[Effect of survivin targeting on cell proliferation and apoptosis in pancreatic cancer cells].

Objective: To investigate the effects of antisense survivin-Lip compound on pancreatic cancer cell proliferation and apoptosis and its mechanism.

Methods: Pancreatic cancer cells of the line PANC-1 were cultured. Survivin oligonucleotide (ODN) was transfected into the PANC-1 cells mediated by Lip reagent.

Expression level of wild-type survivin in gastric cancer is an independent predictor of survival.

Aim: Survivin is a novel antiapoptotic gene in which three splicing variants have been recently cloned and characterized. Survivin has been found to be abundantly expressed in a wide variety of human malignancies, whereas it is undetectable in normal adult tissues. We aimed to study the expression of three survivin splicing variants in gastric cancer, and to evaluate the prognostic significance of the expression of survivin variants in gastric cancer.

Effects of daidzein on estrogen-receptor-positive and negative pancreatic cancer cells in vitro.

Aim: To study the effects of daidzein on human pancreatic cancer cells in vitro.

Methods: Human estrogen-receptor (ER)-positive pancreatic cancer cells MiaPaCa-2 and ER-negative pancreatic cancer cells PANC-1 were treated by 0.1 micromol/L, 1 micromol/L, 10 microL, 25 microL, 50 microL, 75 microL and 100 microL of daidzein, respectively.