Purpose: The purpose of this research is to detect Newcastle disease virus and to assess the seropositivity among backyard, semi-intensive, and intensive farms located in central and southwestern areas of Ethiopia.
Material And Methods: A total of 239 oropharyngeal and cloacal swab samples were collected from symptomatic birds found in Holeta, Burayu, Jimma towns as well as Seka Chekorsa and Nadhigibe woredas of Jimma Zone. In addition, ninety blood samples were collected from wing veins of unvaccinated birds found in the study areas of Jimma zone.