Preparation and application of a multiepitope fusion protein based on bioinformatics and Tandem Mass Tag-based proteomics technology.

Introduction: Brucellosis is a widespread zoonotic disease that poses a considerable challenge to global public health. Existing diagnostic methods for this condition, such as serological assays and bacterial culture, encounter difficulties due to their limited specificity and high operational complexity. Therefore, there is an urgent need for the development of enhanced diagnostic approaches for brucellosis.

Development and evaluation of the recombinant BP26 protein-based C-ELISA for human brucellosis diagnosis.

Introduction: Timely and accurate diagnosis is crucial for the effective treatment and prevention of brucellosis. Current serological diagnostics, primarily based on lipopolysaccharide (LPS), suffer from cross-reactivity with other Gram-negative bacteria, which limits their specificity. Periplasmic protein 26 (BP26), a highly immunogenic antigen found in , has emerged as a promising alternative for enhancing diagnostic specificity.

College students' psychology and behavior in the context of online public opinion: a cross-sectional study in Jiangsu Province, China.

Background: With the rapid development of the Internet and the widespread use of social media, online public opinion has profoundly impacted the psychology and behavior of college students. College students are in a crucial stage of psychological development and self-awareness, making them highly sensitive to online information and easily influenced by online public opinion.

Methods: This study employed a cross-sectional design to explore the psychological adaptation and behavioral responses of college students to online public opinion.

HIV/AIDS-related knowledge and attitudes toward people living with HIV among college students in Xuzhou, Jiangsu Province, China: a cross-sectional survey.

Background: In the contemporary landscape, college students have emerged as a demographic increasingly vulnerable to AIDS. Recognizing that heightened awareness and progressive attitudes toward HIV are pivotal in its prevention, this study was conceived with the intent to meticulously evaluate the level of HIV understanding amongst college students, gauge their sentiments toward people living with HIV (PLHIV), and scrutinize factors influencing these perceptions.

Method: This study used an anonymous online questionnaire to collect data through a cross-sectional survey.

Development of a colloidal gold immunochromatographic test strip for detecting the smooth Brucella.

Brucellosis, caused by Gram-negative Brucella, spreads in human and animal populations through contact with infected animals and products. Developing a rapid and sensitive detection technology for pathogen is crucial to reduce the risk of this disease transmitting between animal populations and to humans. We produced a monoclonal antibody LPS-6B5, which shows high affinity to LPS and limited cross-reactivity with other bacteria.

Production and evaluation of anti-BP26 monoclonal antibodies for the serological detection of animal brucellosis.

BP26 proves to be a highly immunogenic antigen with excellent specificity in brucellosis detection. In China, the authorized use of the Bp26-deleted vaccine M5ΔBP26 for preventing small ruminant brucellosis highlights the importance of developing accurate detection methods targeting BP26, particularly for the diagnosis of differentiation between infected and vaccinated animals (DIVA). Using the traditional mouse hybridoma technique, we successfully obtained 12 monoclonal antibodies (mAbs) targeting BP26.

Cell phone addiction and sleep disturbance among medical students in Jiangsu Province, China: the mediating role of psychological resilience and the moderating role of gender.

Background: Cell phone addiction presents a widespread and severe physical and mental health concern, now recognized as a global public health issue. Among medical college students, the issue of poor sleep quality has become particularly prevalent. This study aimed to investigate the relationship between cell phone addiction and sleep disturbance in a population of medical college students, exploring the potential mediating role of psychological resilience and the moderating impact of gender.

Prevalence and related factors of sleep quality among Chinese undergraduates in Jiangsu Province: multiple models' analysis.

Background And Aims: In China, a significant number of undergraduates are experiencing poor sleep quality. This study was designed to investigate the prevalence of poor sleep quality and identify associated factors among undergraduates in Jiangsu Province, China.

Methods: A total of 8,457 participants were collected in 2022 using whole-group convenience sampling.

Factors associated with sleep disorders among university students in Jiangsu Province: a cross-sectional study.

Objective: This study aims to establish the precise prevalence of sleep disorders among university students in Jiangsu Province. Utilizing a representative sample of students, we measured their sleep quality based on the Pittsburgh Sleep Quality Index (PSQI). Our objective is to quantitatively assess the magnitude of sleep quality and identify key factors.

Psychological resilience buffers the association between cell phone addiction and sleep quality among college students in Jiangsu Province, China.

Background And Aims: Excessive use of cell phones can take up college students' time and energy, and the sleep quality will inevitably be affected. A high level of psychological resilience can help them to maintain a positive attitude and cope with stressful events. However, few studies were conducted to investigate the effects of psychological resilience buffering cell phone addiction on sleep quality.

Correction: Paper-based ELISA diagnosis technology for human brucellosis based on a multiepitope fusion protein.

[This corrects the article DOI: 10.1371/journal.pntd.

Study on antigenic protein Omp2b in combination with Omp31 and BP26 for serological detection of human brucellosis.

Background: Brucellosis is a very common zoonosis in certain localized areas worldwide, with a high prevalence in most developing countries. The detection of brucellosis still faces many challenges such as the need for more sensitive and specific diagnostic antigens.

Methods: To evaluate the efficacy of Brucella outer membrane proteins (Omps) Omp2b in combination with omp31 and BP26 as diagnostic antigens for the serological detection of human brucellosis, these proteins were prepared by a prokaryotic expression system.

Study on lifestyle habits affecting sleep disorders at the undergraduate education stage in Xuzhou City, China.

Background: In China, undergraduate students face both academic and career selection pressures, sleep is an important physiological process for them. Investigate the physical exercise, sleep quality of undergraduate students in the education stage in Xuzhou City, and analyze the factors affecting their sleep quality, to promote the health education and psychological health of undergraduate students.

Materials And Methods: The Physical Activity Rating Scale-3 (PARS-3), the Pittsburgh Sleep Quality Index (PSQI), and the demographic information questionnaire were used to survey a whole-group sample of four undergraduate colleges and universities (Xuzhou Institute of Engineering, Xuzhou Medical University, China University of Mining and Technology, Jiangsu Normal University) in Xuzhou by cluster sampling, the general characteristics including gender, grade, height, weight, domicile, race, economic income, etc.

Comparison of BP26, Omp25 and Omp31 and a Multiepitope-Based Fusion Protein in the Serological Detection of Canine Brucellosis.

Background: Brucellosis is one of the most important zoonotic diseases in the world. Canine brucellosis, caused mainly by , is seriously neglected, and there is a lack of accurate diagnostic tools.

Methods: In this study, to compare BP26, Omp25, Omp31 and a multiepitope-based fusion protein in the serological detection of canine brucellosis, using 34 brucellosis-positive dog sera and 62 negative control sera, the outer membrane proteins Omp31, BP26, Omp25 and a multiepitope-based fusion protein were evaluated by iELISA for their potential use as antigens in the serological diagnosis of canine brucellosis.

Evaluation of the Combined Use of Major Outer Membrane Proteins in the Serodiagnosis of Brucellosis.

Background: Brucellosis is a zoonotic disease that causes substantial public health problems and endangers the development of animal husbandry in endemic areas. Early diagnosis of infected animals and humans is a crucial step in reducing the incidence of brucellosis. In this study, we designed different combinations of major outer membrane proteins (omps) including omp10, omp16, omp19, omp25, omp31 and BP26 as antigens and evaluated their efficiency in serodiagnosis for brucellosis.

Estimation of Plasmodium falciparum transmission using multiepitope chimeric antigen in the postelimination phase in Yunnan, China.

Background: The continuous monitoring of malaria transmission intensity is still required to maintain elimination status after reaching the malaria elimination stage. In this study, serological surveillance with multiepitope artificial antigen was used to assess the transmission of Plasmodium falciparum in Yunnan, China, where malaria elimination has just been achieved, to provide data to support malaria control in the postelimination period.

Methods: Samples were collected in three border counties and one inland county in Yunnan Province in 2016 using a stratified whole-group sampling method.

Surveillance of Plasmodium vivax transmission using serological models in the border areas of China-Myanmar.

Background: To understand the Plasmodium vivax malaria transmission intensity and to assess the effectiveness of prevention and control measures taken along the China-Myanmar border, a catalytic model was used to calculate the seroconversion rate, an important indicator of malaria transmission intensity with high sensitivity, which is particularly useful in areas of low transmission.

Methods: Five counties in Yunnan Province bordering Myanmar were selected as survey sites, and subjects were obtained in each county by stratified random sampling in 2013-2014. Fingerstick blood was collected from each subject and tested for antibodies to P.

Lance Adams syndrome: two cases report and literature review.

Hypoxic myoclonus, also known as Lance Adams syndrome, is a rare syndrome that results from the serious brain damage caused by cerebral hypoxia that often follows cardiopulmonary resuscitation. This current case report describes two patients with post-hypoxic myoclonus, both of whom received cardiopulmonary resuscitation. The neurological symptoms of these two patients were significantly improved by the administration of clonazepam and sodium valproate sustained-release tablets.

A Multi-Epitope Fusion Protein-Based p-ELISA Method for Diagnosing Bovine and Goat Brucellosis.

In recent years, the incidence of brucellosis has increased annually, causing tremendous economic losses to animal husbandry in a lot of countries. Therefore, developing rapid, sensitive, and specific diagnostic techniques is critical to control the spread of brucellosis. In this study, bioinformatics technology was used to predict the B cell epitopes of the main outer membrane proteins of , and the diagnostic efficacy of each epitope was verified by an indirect enzyme-linked immunosorbent assay (iELISA).

Paper-based ELISA diagnosis technology for human brucellosis based on a multiepitope fusion protein.

Background: Brucellosis, as a serious zoonotic infectious disease, has been recognized as a re-emerging disease in the developing countries worldwide. In china, the incidence of brucellosis is increasing each year, seriously threatening the health of humans as well as animal populations. Despite a quite number of diagnostic methods currently being used for brucellosis, innovative technologies are still needed for its rapid and accurate diagnosis, especially in area where traditional diagnostic is unavailable.

Comparative analysis of the main outer membrane proteins of Brucella in the diagnosis of brucellosis.

Brucellosis has placed a heavy economic burden on numerous countries and has consumed considerable medical resources worldwide. To improve the specificity and sensitivity of serological methods for diagnosing brucellosis, it is important to develop new diagnostic antigens. Brucella outer membrane proteins(omps) possess good immunogenicity, but there is a scarcity of comparative studies of these proteins in the clinical diagnosis of brucellosis.

Suoquan pill for the treatment of diabetic nephropathy: A protocol for systematic review and meta-analysis.

Background: Diabetic nephropathy (DN) is one of the most common complications of diabetes and the main cause of kidney failure in developed countries. Clinically, DN is usually treated by controlling blood sugar and blood pressure. According to reports, the application of angiotensin-converting enzyme inhibitor (ACEI) or angiotensin receptor antagonist can only target a certain stage of disease development.

Study on immunogenicity and antigenicity of a novel brucella multiepitope recombined protein.

Currently, brucellosis is a reemerged zoonotic infectious disease with an increased incidence in recent years. A simple, rapid and sensitive method for diagnosing brucellosis can help to reduce medical burden and economic loss. Previously, a multiple epitope recombinant protein was constructed based on linear B-cell epitope prediction tools.

A novel recombinant multiepitope protein candidate for the diagnosis of brucellosis: A pilot study.

Brucellosis is a zoonotic disease transmitted to humans from infected animals. As a systemic disease, it can harm any organ or system of the host body. Human brucellosis presents with various clinical symptoms, which makes diagnosis challenging.

Predicting Diabetes Mellitus With Machine Learning Techniques.

Diabetes mellitus is a chronic disease characterized by hyperglycemia. It may cause many complications. According to the growing morbidity in recent years, in 2040, the world's diabetic patients will reach 642 million, which means that one of the ten adults in the future is suffering from diabetes.