[Development and evaluation of a rapid PCR detection kit for Ophiocordyceps sinensis].

Ophiocordyceps sinensis is a valuable traditional Chinese medicine. Due to resource shortage, expensive price and huge market demand, there are many adulterants of O. sinensis in markets.

[Correlation analysis between effectiveness of element in rhizonsphere soil and quality of Danshen (Salvia miltiorrhiza)].

In order to investigate the content and distribution of available element in the rhizonsphere soil of the growing areas of Salvia miltiorrhiza Bunge, the contents of available element (N,P,K,B,Cu,Zn,Fe,Mn) in 26 soil samples were tested and evaluated. The results showed that the contents of available P and Fe were very plentiful, available K, Cu and Zn were rich, available N and Mn were deficient, available B was extremely deficient in all growing areas of S. miltiorrhiza of eight provinces in China.

[Study on quality assessment of Polygalae Radix based on HPLC-DAD fingerprint].

Objective: To establish an HPLC fingerprint to evaluate the quality of Polygalae Radix, root xylem, and those collected in different growth ages or harvest time.

Method: Separation was performed at 30 °C on a Kromasil C18 column (4.6 mm x 250 mm, 5 μm); the mobile phases was acetonitrile and 0.

[Preliminary study on correlation between diversity of soluble proteins and producing area of Cordyceps sinensis].

To analyze the content and type of soluble proteins in Cordyceps sinensis from different producing areas and processed with different methods with bradford method and 2-DE technology, in order to discover significant differences in soluble proteins in C. sinensis processed with different methods and from different producing areas. The preliminary study indicated that the content and diversity of soluble proteins were related to producing areas and processing methods to some extent.

[Market survey and identification of hippocampus (Haima)].

Objective: To clarify the commercial original species of hippocampus in the market.

Method: Field survey and interview were applied to the investigation.

Result: Present study identified the main commercial hippocampus of 13 species, including Pharmacopoeia contained four kinds of hippocampus (without Hippocampus japonicus) listed in Chinese Pharmacopoeia, and the identification method for the 13 kinds of commercially hippocampus was established.

[Corresponding relationship between Mantis and Mantidis Oötheca (Sangpiaoxiao)].

Mantidis Oötheca is commonly used Chinese medicine. Because of the used medicinal part is oötheca and many mantis species can yield ootheca, it is not possible to identify its original animal accurately. There is no unanimous conclusion about the corresponding relationship between Mantis and Mantidis Oötheca (Sangpiaoxiao).

[Effects of different harvest times and processing methods on quality of Gardeniae Fructus].

Objective: To study effects of different harvest times and processing methods on the quality of Gardeniae Fructus.

Method: The content of asminoidin and the similarity of the fingerprint of Gardeniae Fructus were determined and applied for assessment of the quality.

Result: Gardeniae Fructus harvested in October with yellow-green appearance was the better time point for harvest.

[Data distribution characteristics and deviation of tanshinone IIA content in Radix Salviae Miltiorrhizae].

Objective: To analyze the content of tanshinone IIA in Radix Salviae Miltiorrhizae from different habitats are regularly altered and find out the deviation between the studies.

Method: The data of the content of tanshinone IIA in samples of Radix Salviae Miltiorrhizae from different areas and related information since 1997 was collected and statistically analyzed.

Result And Conclusion: The content of tanshinone IIA in wild Salvia was generally higher than that in the cultivated Salvia, and the content in nearly half cultivated Salvia was less than 0.

[Characteristics of soil microbial variation during crop rotation period at cultivation area of Salvia miltiorrhiza in Zhongjiang of Sichuan province].

Objective: To study the characteristics of soil microbial variation during Salvia miltiorrhiza crop rotation.

Method: the conventional cultivating microbial method was used to study the microbial number and communities structure and soil microbial biomass phosphorus (SMBP) was determined by chloroform vapor extraction method. The data was then analyzed by SPSS software.

[GC-MS fingerprint analyses of fatty acids in peach seed].

Objective: To investigate the GC fingerprint of fatty acids in peach seed,and provide evidences for quality assessment on peach seed.

Method: Capillary gas chromatography was applied in analysis of fatty acids in peach seed, and the distinctive information was confirmed by GC-MS with hierarchical cluster and species similarity analysis.

Result: The GC fingerprint containing distinctive information was obtained after investigation of peach seeds from 34 varieties and origins.

[Resources of Chinese Herbs Sang (Morus sp.) in Sichuan and strategies for development and utilization].

A comprehensive analysis was conducted on resources composition of Chinese Herbs Sang (Morus sp.) in Sichuan using survey data and related literature. The original plants, germplasm collections, cultivation areas, main cultivated varieties and production sale of crude drugs of Sang in Sichuan were clearly expounded.

[Principal component and cluster analysis of trace elements in Chinese herb Salvia miltiorrhiza and its relative species].

The contents of trace elements, including copper, zinc, iron, magnesium, calcium, chromium, lead, molybdenum, manganese, and cadmium in Chinese traditional herb S. miltiorrhiza and its relative species such as S. miltiorrhiza f.

[Isolation and identification of endophytic fungi from Ligusticum].

Objective: To investigate the correlation between entophytic fungal community, habitations and varieties of Ligusticum.

Method: The solidified potato dextrose agar (PDA) of plates was applied for the isolation of the endophytic fungi, and the identification was completed by spot-planting method.

Result: Fifty strains of the entophytic fungi were isolated from the rhizome of L.

[Determination of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae].

Objective: To determine the contents of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae in Sichuan province and Chongqing city for evaluating the quality of them.

Methods: The HPLC analysis and spectrophotometry analysis were used.

Results: The contents of chlorogenic acid and total caffeoylquinic acid in 6 Folium Lonicerae had been determined.

Effects of tenuifolin extracted from radix polygalae on learning and memory: a behavioral and biochemical study on aged and amnesic mice.

Although normal cognitive changes take place when a person becomes older, aging in humans is generally associated with deterioration of cognitive performance and, in particular, of learning and memory. These cognitive deficits can cause debilitating consequences due to aging. There are a number of herbal medicines which are reported to improve brain function including intelligence.

[Study on relative bioavailability of ferulic acid of combination Radix Angelicae Sinensis and Cortex Cinnamomi in mice].

Objective: To study the phamacokinetics and relative bioavailability of combination Radix Angelicae Sinensis and Cortex Cinnamomi.

Method: The content of ferulic acid in plasma was determined directly by HPLC after oral administration of Cortex Cinnamomi in combination individually with Radix Angelicae Sinensis. The plasma concentration-time curve were plotted.

[HPLC fingerprint of Rhizoma Polygoni Cuspidati].

Objective: To establish the HPLC fingerprint of Rhizoma Polygoni Cuspidati (Polygonum cuspidatum).

Method: The HPLC separation was carried with Diamonsil C18 column and eluted with a gradient from methanol and 0.1% phosphoric acid, the detection wavelength was at 230 nm and recording 70 min.

Pharmacological study on Samia cynthia ricini in old female mice.

The effect of the extraction from female moths Samia cynthia ricini (family Saturniidae) on menopausal syndrome was studied in order to search for an effective traditional Chinese medicine to menopausal syndrome and offer a theoretical support for the further research on Samia cynthia ricini. Aged, nonreproductive female mice was used as the model and randomly divided into three groups: control, ethanol extraction group and diethylstilbestrol (DES) group according to the medicine applied. After 4 weeks of treatment, keratinization rates of vagina epithelia, indexes of organs, serum concentrations of estradiol (E2) and progesterone (P), the bone mineral contents (BMC) and the morphological changes of ovary and uterus were measured.

[Study on enhancing bioavailability of paeoniflorin by combined use with Chinese herbs for inner-warming].

Objective: To study the influence of Chinese herbs for inner-warming on the bioavailability of paeoniflorin (PF) and its mechanism.

Methods: Chinese herbs (pepper fruit, evodia fruit, cassia bark, fennel fruit and prickly-ash peel) were separately used in combination with PF for gastrogavage to mice. Reversed phase high-performance liquid chromatography was used to determine the plasma concentration of PF in mice after medication.

[Determination of hyperin in genus Hypericum medicinal plants].

Objective: To compare the content of hyperin in Hypericum medicinal plants.

Methods: HPLC method was established. Alltima C18 radial compression column was used with a mobile phase of methanol-0.