Post-operative effects on insulin resistance and specific tension of single human skeletal muscle fibres.

Surgery and accidental trauma are associated with a transient period of insulin resistance, substrate catabolism and muscle weakness. In the present study, we evaluated the changes in the force-generating capacity of chemically skinned single muscle fibres following abdominal surgery. Biopsies of the m.

Age-related changes in the microcirculation of skeletal muscle.

The age-related reduction in exercise capacity is associated with a reduction in cardiac output and maximal oxygen consumption (VO2max). The loss of muscle mass explains a large portion of the age-related decline in VO2max. The capillary supply to a muscle fibre is primarily determined by its size, but also by its metabolic profile and the metabolic profile of surrounding fibres.

Gender- and age-related differences in the regulatory influence of thyroid hormone on the contractility and myosin composition of single rat soleus muscle fibres.

The effects of 4 weeks of thyroid hormone (3,5,3'-triiodothyronine, T3) treatment on the myosin isoform composition and maximum velocity of unloaded shortening (V0) of single soleus muscle fibres of young (3-6 months) and old (20-24 months) female (149 fibres) and male (200 fibres) rats were studied. Gender-related differences in the up-regulation of fast myosin heavy chain (MyHC) and myosin light chain (MyLC) isoforms were observed. In the female hyperthyroid rats, pure type I fibres and fibres co-expressing type I and type IIA MyHC (type I/IIA fibres) predominated.

Morphological and functional evidence, and clinical importance, of vascular anastomoses in the latissimus dorsi muscle of the sheep.

Mobilisation of the latissimus dorsi muscle as a functional graft necessarily involves division of perforating arteries that enter the distal portion of the muscle, rendering it vulnerable to ischaemic damage when the muscle is stimulated electrically. Using a fluorescent microsphere technique we showed that the blood flow contributed by the thoracodorsal artery decreases in a proximal-to-distal direction, and that of the perforating arteries in a distal-to-proximal direction, but for neither does the flow decline to zero. This is consistent with earlier reports of anastomotic connections between the 2 arterial territories.

Intramuscular pressure, force and blood flow in rabbit tibialis anterior muscles during single and repetitive contractions.

The elevated intramuscular pressure (IMP) associated with sustained muscle contraction can affect blood flow, and could influence the long-term viability of functional skeletal muscle grafts. We therefore examined the relationship between force, peak IMP and blood flow in the tibialis anterior muscle of the anaesthetized rabbit. During isometric contractions.

Effects of age and gender on shortening velocity and myosin isoforms in single rat muscle fibres.

The maximum velocity of unloaded shortening (V0) and the myosin heavy chain (MyHC) and light chain (MyLC) isoform composition were determined in single fibres from soleus and extensor digitorum longus (EDL) muscles of male and female rate 3-6 and 22-24 months old. In the soleus muscle, the beta/slow (type I MyHC) isoform predominated in both young and old animals, irrespective of gender. In the EDL, fibres expressing type IIX MyHC or a combination of IIX and IIB (IIXB) MyHC isoforms were predominant in old rats, while type IIB MyHC fibres predominated in young individuals of both genders.

Intramural blood flow of skeletal muscle ventricles functioning as aortic counterpulsators.

Background: Skeletal muscle ventricles (SMVs) working as aortic counterpulsators have provided long-term left ventricular assistance under experimental conditions. However, gradual deterioration of SMV pump function and rupture have been observed, and this may be related to compromised intramural blood flow during synchronized counterpulsation under systemic working conditions.

Methods: Transformed, double-layered SMVs in 6 sheep were stimulated for 3-minute periods (5 V, 30 Hz, burst duration and delay from QRS both 40% of the cardiac cycle) to work as diastolic counterpulsators in the systemic circulation at a 1:2 (SMV:heart) and 1:1 ratio, and on a mock circulation with low-pressure loading conditions at a 1:2 ratio.

The use of coloured dye-extraction microspheres to measure blood flow in rabbit skeletal muscle: a validation study with special emphasis on repeated measurements.

We evaluated the use of coloured dye-extraction microspheres for measuring blood flow in the skeletal muscle of anaesthetized rabbits. Spheres were injected into the left atrium after sternotomy or lateral thoracotomy, or into the left ventricle via the left carotid artery. Both routes of administration produced adequate mixing of microspheres with the blood.

The development of compensatory hypertrophy in the plantaris muscle of the rat.

The aim of this investigation was to study the time course of compensatory hypertrophy (CH) over a seven week period after its surgical induction in the lower limb of the rat. CH of the left plantaris muscle of the rat was induced by denervation of the ipsilateral gastrocnemius and soleus muscles. Muscle fibres were classified as type I, Ic, IIa and IIb.

Specific force of the rat plantaris muscle changes with age, but not with overload.

Age and overload effects on the specific force of the rat plantaris muscle were investigated. The specific force was affected by age, but not by overload, inducing a 30% hypertrophy, at any age. The relative amount of non-contractile tissue only minimally affected the conclusions.

Determination of oxygen consumption in muscle during exercise using near infrared spectroscopy.

The aim of this study was to determine oxygen consumption (VO2) during isometric exercise in human muscles using near infrared spectroscopy (NIRS). The technique was used to study the relationship between VO2 in the soleus muscle and the level of isometric exercise expressed as percentages of the maximum voluntary contraction (MVC). For the study 11 healthy male volunteers were recruited.

Changes in oxidative capacity and fatigue resistance in skeletal muscle.

In conclusion, it appears that in general an increase in the fatigue resistance of a muscle is accompanied by an increase in its oxidative capacity. Fatigue resistance of a muscle seems to be partly determined by its oxidative capacity. On the single motor unit (Burke et al, 1973; Hamm et al, 1988; Kugelberg and Lindegren 1979; Larsson et al, 1991) and single fibre level (Nemeth et al, 1981) the relation between fatigue resistance and oxidative capacity seems to be valid.

Capillarisation and fibre types in hypertrophied m. plantaris in rats of various ages.

Influences of age, overload obtained through denervation of synergists, and training on the capillarisation of the m. plantaris were compared in 5-, 13- and 25-month-old rats in relation to different fibre types. Overload resulted in about 30% hypertrophy in each age group.

Metabolic capacity, fibre type area and capillarization of rat plantaris muscle. Effects of age, overload and training and relationship with fatigue resistance.

1. The influences of age (5, 13 and 25-month-old rats), overload as obtained by denervation of synergists, and training on the metabolic capacity, relative muscle cross-sectional area occupied by each fibre type, capillarization and fatigue resistance of the rat m. plantaris were investigated.

Compensatory hypertrophy and training effects on the functioning of aging rat m. plantaris.

The influence of age, compensatory hypertrophy and training on isometric contraction characteristics of rat m. plantaris were investigated in 5-, 13- and 25-month-old rats. Each age group was subdivided into Control Not Trained, Control Trained, Operated Not Trained and Operated Trained groups.

The relationship between capillarisation and fibre types during compensatory hypertrophy of the plantaris muscle in the rat.

Compensatory hypertrophy of the plantaris muscle was obtained by denervation of its synergists. This hypertrophy is characterised by a 32% increase in muscle mass. The muscle consists of type I and IIa (oxidative), and IIb (glycolytic) fibres.