Publications by authors named "Defu Chi"

Article Synopsis
  • - The study focuses on the Lepidoptera pest Denis Schiffermuller, which harms Pinaceae plants, and analyzes the midgut transcriptome of larvae infected with strain 2913 over various time intervals, identifying 7497 differentially expressed genes (DEGs) related to the infection.
  • - Among these DEGs, several genes linked to toxin activation, receptor proteins for insecticidal proteins, and metabolic detoxification were highlighted, suggesting a complex response mechanism in the larvae to counteract infection over time.
  • - The research indicates that enzymes like trypsin and chymotrypsin play roles in activating and degrading toxins, while certain protein expressions may either enhance or weaken the larvae's resistance to the pest strain
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The lignin, cellulose and hemicellulose contained in corn straw need to be degraded before reuse. Therefore, it is urgent to explore a new strategy that can enhance the degradation effect of lignin, cellulose and hemicellulose. is one of the corn pests feeding on corn straw, which can degrade and consume corn straw by digestive enzyme secreted in the midgut.

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The genus contains several destructive borer pests that are found in coniferous forests in the Northern Hemisphere. spore powder was tested as a new method of pest control. In this study, (Lepidoptera: Pyralidae) was used as the object.

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Korean pine is an economically essential afforestation species limited by the unreasonable collection of cones, indiscriminate use of chemical pesticides and pest damage. This study aimed to determine whether spraying bacterial or fungal solutions affected insect pests, cone development, and the seed quality of Korean pine Sieb. et Zucc.

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Dioryctria abietella is a coniferous seed orchard pest that can damage a series of host plants and cause huge losses to the forest economy. Sex pheromones play an important role in lepidopteran sex communication for reproduction and can be used as biological control agents to monitor and trap pests. However, the genes involved in the biosynthesis, transportation, and degradation of D.

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The coneworm Dioryctria abietella (Lepidoptera: Pyralidae) is an economy devastating pest that infests many valuable conifer species in the Holarctic regions, such as Pinus koraiensis Siebold and Zucc. The chemosensory system plays a crucial role in the mating, foraging, and ovipositing of this pest, and therefore it is desirable to identify chemosensory molecules for pest control. However, little is known at molecular level about the olfactory mechanisms in D.

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The gray tiger longicorn beetle, Linnaeus (Coleoptera: Cerambycidae) is a stem-boring pest that can inhibit not only the transportation of nutrients in the trunk but also the tree growth, increasing the risk of tree breakage and causing economic losses. It is distributed in China, Iran, Turkey, Russia, Korea, Japan, and Southern Europe. This study aimed to investigate selected strains that could be used as entomopathogenic fungi for the biological control of this pest.

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D. Don is a medicinal plant rich in 20-hydroxyecdysone (20E), alkaloids, and other active substances. In this study, the cell suspension was incubated for 7 days, followed by the analysis on the effects of abscisic acid (ABA) on the regulation of 20E synthesis.

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Bunge cells contain β-ecdysterone (β-EC) that regulates the molting process of insect larvae. In this study, different conditions of culture have been studied to optimize the production of β-EC. Bunge growth fitted the curve of logistic equation with one growth cycle of 17 days.

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Suspension culture of Ajuga lobata D. Don cells provides a method of synthesis of the phytoecdysteroid 20-hydroxyecdysone (20E) which can regulate the molting process of larvae. We characterized the culture conditions to optimize 20E production.

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Stable and even microcrystals of Avermectin (AVM) were produced by recrystallization in presence of a stabilizer. Sequential layer growth was achieved by the layer-by-layer (LbL) self-assembly of biocompatible polyelectrolytes (PEs). The coated colloids were characterized using confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM).

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A total of 973 isolates of endophytic fungi were recovered from 1,144 tissue fragments of the six medicinal plant species belonging to 4 families collected in the Beijing Botanical Garden. Of these isolates 778 sporulated and were identified into 21 taxa by morphological characteristics. Among the taxa 11 belonged to Coelomycetes, 6 to Ascomycetes, and 4 to Hyphomycetes.

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With RAPD-PCR technique, the phylogenetic tree of 16 olethreutid moth species in northeast China was constructed, and their consanguinity was analyzed. The results showed that the clustering of 3 Pandemis species,2 Argyrotaenia species and 4 Archips species on this phylogenetic tree was coincident with their morphological taxology, but that of some other species was not. For example, Clepsis rurinana and C.

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This paper summarized the application of RAPD techniques in insect taxology and ecology, relative of species, systemic development, identification of pest, diagnosing for pest resistance, construction of molecular linkage map, and molecular assistant breeding. The problems existing in the applications of RAPD in entomology were indicated, and their countermeasures were given. It was mentioned that with the development of theories and experimental techniques, insect molecular biology would make new progress and conquer all those problems.

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Plant volatiles show different effects on insect behaviors, which included luring, repelling and killing. After damaged by phytophagous insects, some host plants could send volatiles luring natural enemies as an indirect defense. A summarize on the new advances of plant volatiles was given.

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