Using preimplantation genetic testing for monogenic disease for preventing citrullinemia type 1 transmission.

Aim: The aim of this study is to investigate if Preimplantation Genetic Testing (PGT) can effectively identify unreported variants according to American College of Medical Genetics and Genomics (ACMG)to prevent citrullinemia type 1 affection.

Design: This study involves a detailed case analysis of a family with history of citrullinemia type 1, focusing on the use of PGT for monogenic diseases (PGT-M). The genetic variants were identified using ACMG guidelines, and PGT was employed to prevent the inheritance of these variants.

Higher serum Lp-PLA2 is associated with cognitive impairment in Parkinson's disease patients.

Objective: To explore the association between lipoprotein-associated phospholipase A2 (Lp-PLA2) and the risk of cognitive impairment in Parkinson's disease (PD-CI).

Methods: A case-control study involving 100 hospitalized PD patients and 60 healthy controls was carried out. Serum Lp-PLA2 level was detected by automatic biochemical analyzer.

The Arabidopsis IDD14 transcription factor interacts with bZIP-type ABFs/AREBs and cooperatively regulates ABA-mediated drought tolerance.

The INDETERMINATE DOMAIN (IDD) transcription factors mediate various aspects of plant growth and development. We previously reported that an Arabidopsis IDD subfamily regulates spatial auxin accumulation, and thus organ morphogenesis and gravitropic responses. However, its functions in stress responses are not well defined.

The assessment on impact of essential drugs policy on primary health care system in rural areas of Shandong Province policy and regulation division of the Health Department of Shandong Province.

At present, China has achieved an initial establishment and gradual implementation of a framework for national essential drugs policy. With the further implementation of the national essential drugs policy, it is not clear how the policy works, whether it achieves the original intention of essential drugs policy, and what impact essential drugs policy exerts on the primary health care system. In view of it, we conducted a field research on sample areas of Shandong Province to understand the conditions of the implementation of the essential drugs policy in Shandong Province.

Comparison of clinical outcomes between fresh embryo transfers and frozen-thawed embryo transfers.

Background: Advances in embryo culture technology and cryopreservation have led to a shift in in vitro fertilization (IVF) from early fresh or frozen-thawed cleavage embryo transfer to fresh or frozen-thawed blastocyst stage transfer.

Objective: To compare the clinical outcomes of fresh embryo transfers and frozen-thawed embryo transfers.

Materials And Methods: In this retrospective case control study, patients undergoing IVF cycles from January 2012 to December 2012 were enrolled in Assisted Reproduction of Wuhan Union Hospital were enrolled.

The role of acupuncture in in vitro fertilization: a systematic review and meta-analysis.

Background/aims: In recent years, acupuncture has become more and more popular in the management of subfertility. The aim of this study was to evaluate the impact of acupuncture during in vitro fertilization (IVF) treatment on the outcomes of clinical pregnancy in published randomized studies.

Methods: This is a systematic review and meta-analysis.

The differential transcription network between embryo and endosperm in the early developing maize seed.

Transcriptome analysis of early-developing maize (Zea mays) seed was conducted using Illumina sequencing. We mapped 11,074,508 and 11,495,788 paired-end reads from endosperm and embryo, respectively, at 9 d after pollination to define gene structure and alternative splicing events as well as transcriptional regulators of gene expression to quantify transcript abundance in both embryo and endosperm. We identified a large number of novel transcribed regions that did not fall within maize annotated regions, and many of the novel transcribed regions were tissue-specifically expressed.

Comparison of the Saint Louis University Mental Status Examination, the Mini-Mental State Examination, and the Montreal Cognitive Assessment in detection of cognitive impairment in Chinese elderly from the geriatric department.

Introduction: The Saint Louis University Mental Status (SLUMS) examination is a popular screening scale for cognitive impairment in North America but has not been studied in Chinese populations. The aim of this study is to compare consistency of the SLUMS with the Chinese version of Mini-Mental Status Examination (CMMSE) and the Beijing version of Montreal Cognitive Assessment (MoCA-B) in Chinese elderly.

Methods: Two hundred seventy-eight Chinese elderly with chronic diseases and geriatric syndromes from the geriatrics department were screened for cognitive impairment, including dementia and mild cognitive impairment, using SLUMS, CMMSE, and MoCA-B.

Antisense-mediated depletion of tomato chloroplast glutathione reductase enhances susceptibility to chilling stress.

A tomato (Lycopersicon esculentum Mill.) chloroplast glutathione reductase gene (LeGR) was isolated and antisense transgenic tomato lines were obtained. Under chilling stress, transgenic plants accumulated more H(2)O(2), leaked more electrolyte and showed lower net photosynthetic rate (Pn), maximal photochemical efficiency of PSII (Fv/Fm) and oxidizable P700 compared with wild-type (WT) plants.

Over-expression of StAPX in tobacco improves seed germination and increases early seedling tolerance to salinity and osmotic stresses.

Ascorbate peroxidase plays a key role in scavenging reactive oxygen species under environmental stresses and in protecting plant cells against toxic effects. The Solanum lycopersicum thylakoid-bound ascorbate peroxidase gene (StAPX) was introduced into tobacco under the control of the cauliflower mosaic virus 35S promoter. Transformants were selected for their ability to grow on medium containing kanamycin.