Metabolomic profiling of dengue infection: unraveling molecular signatures by LC-MS/MS and machine learning models.

Background & Objective: The progression of dengue fever to severe dengue (SD) is a major public health concern that impairs the capacity of the medical system to predict and treat dengue patients. Hence, the present study used a metabolomic approach integrated with machine models to identify differentially expressed metabolites in patients with SD compared to nonsevere patients and healthy controls.

Methods: Comprehensively, the plasma was collected at different clinical phases during dengue without warning signs (DWOW, N = 10), dengue with warning signs (DWW, N = 10), and SD (N = 10) at different stages [i.

Understanding COVID-19 outcome: Exploring the prognostic value of soluble biomarkers indicative of endothelial impairment.

Host proteins released by the activated endothelial cells during SARS-CoV-2 infection are implicated to be involved in coagulation and endothelial dysfunction. However, the underlying mechanism that governs the vascular dysfunction and disease severity in COVID-19 remains obscure. The study evaluated the serum levels of Bradykinin, Kallikrein, SERPIN A, and IL-18 in COVID-19 (N-42 with 20 moderate and 22 severe) patients compared to healthy controls (HC: N-10) using ELISA at the day of admission (DOA) and day 7 post-admission.

Urinary Metabolomics Identified Metabolic Perturbations Associated with Gutka, a Smokeless Form of Tobacco.

Gutka, a form of smokeless tobacco, is widely used in the Indian subcontinent and in other regions of South Asia. Smokeless tobacco exposure is most likely to increase the incidence of oral cancer in the Indian population, and metabolic changes are a hallmark of cancer. The development of biomarkers for early detection and better prevention measures for smokeless tobacco users at risk of oral cancer can be aided by studying urinary metabolomics and offering insight into altered metabolic profiles.

Integrated metabolomics and machine learning approach to predict hypertensive disorders of pregnancy.

Background: Hypertensive disorders of pregnancy account for 3% to 10% of maternal-fetal morbidity and mortality worldwide. This condition has been considered one of the leading causes of maternal deaths in developing countries, such as India.

Objective: This study aimed to discover hypertensive disorders of pregnancy-specific candidate urine metabolites as markers for hypertensive disorders of pregnancy by applying integrated metabolomics and machine learning approaches.

Cost-effectiveness of open versus arthroscopic rotator cuff repair.

Background: Economic evaluation of surgical procedures is necessary in view of more expensive newer techniques emerging in an increasingly cost-conscious health care environment. This study compares the cost-effectiveness of open rotator cuff repair with arthroscopic repair for moderately size tears.

Materials And Methods: This was a prospective study of 30 consecutive patients, of whom 15 had an arthroscopic repair and 15 had an open procedure.

Compression forces generated by Mini bone screws--a comparative study done on bone model.

The compressive forces generated by the AO/ASIF 3.0 mm cannulated cancellous and 2.0 mm cortical screws, Mini-Acutrak and Herbert/Whipple small bone cannulated screws were measured in the laboratory with the use of simulated cancellous bone and a load cell washer as a means of quantifying their fixation capabilities.

Upper-extremity deep-vein thrombosis following a clavicular fracture.

Upper-extremity deep-vein thrombosis (DVT) is not frequently reported in orthopaedic literature. We report a case of upper-limb DVT following an acute clavicular fracture that was treated conservatively with a figure-of-eight sling. Diagnosis of this condition requires a high index of suspicion, and treating it would prevent a potentially fatal pulmonary embolism.