Publications by authors named "Deepak S Jagadeesh"
Article Synopsis
- - The study investigated Staphylococcus aureus in milk, finding a 34.4% prevalence, with 27% identified as methicillin-resistant (MRSA) and 24.71% showing multiple antimicrobial resistance (MAR) patterns.
- - The isolates tested positive for enterotoxin genes sea and sed, and exhibited significant biofilm formation, indicating a potential health risk associated with contaminated milk.
- - The research highlights the diverse genetic profiles of S. aureus and calls for ongoing monitoring and strategies to enhance food safety in Chennai, given its implications for public health.
We isolated and characterized two lytic bacteriophages against named TANUVAS_MVC-VPHSA1 and TANUVAS_MVC-VPHSA2, with the aim of investigating their genomic and structural features. The bacteriophages belong to the Caudoviricetes, and their genomes have sizes of 50,505 and 50,516 base pairs with a GC content of 41.4%.
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- Milk is a nutrient-rich food that can contain harmful microorganisms, particularly a dangerous foodborne pathogen linked to infections and antimicrobial resistance (AMR) in animals.
- A study in Chennai, India, analyzed 259 raw milk samples from dairy farms, local vendors, and retail outlets, finding 34% positive for the pathogen, with significant genetic diversity among the isolates.
- The research noted that all isolates were resistant to ampicillin and identified 28 multidrug-resistant isolates, highlighting worries about food safety and the economic impact of AMR in milk sources.
Background: Fraudulent mislabelling of processed meat products on a global scale that cannot be detected using conventional techniques necessitates sensitive, robust and accurate methods of meat authentication to ensure food safety and public health. In the present study, we developed an in-gel (two-dimensional gel electrophoresis, 2DE) and OFFGEL-based proteomic method for authenticating raw and cooked water buffalo (Bubalus bubalis), sheep (Ovis aries) and goat (Caprus hircus) meat and their mixes.
Results: The matrix-assisted liquid desorption/ionization time-of-flight mass spectrometric analysis of proteins separated using 2DE or OFFGEL electrophoresis delineated species-specific peptide biomarkers derived from myosin light chain 1 and 2 (MLC1 and MLC2) of buffalo-sheep-goat meat mix in definite proportions at 98:1:1, 99:0.
The present study compared the accuracy of an OFFGEL electrophoresis and tandem mass spectrometry-based proteomic approach with a DNA-based method for meat species identification from raw and cooked ground meat mixes containing cattle, water buffalo and sheep meat. The proteomic approach involved the separation of myofibrillar proteins using OFFGEL electrophoresis, SDS-PAGE and protein identification by MALDI-TOF MS. Species-specific peptides derived from myosin light chain-1 and 2 were identified for authenticating buffalo meat spiked at a minimum 0.
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