Oxidative Folding Catalysts of Conotoxins Derived from the Venom Duct Transcriptome of and .

Two novel redox conopeptides with proline residues outside and within the active site disulfide loop were derived from the venom duct transcriptome of the marine cone snails and . Mature peptides with possible post-translational modification of 4-trans-hydroxylation of proline, namely, Fr874, Fr890[P1O], Fr890[P2O], Fr906, Am1038, and Am1054, have been chemically synthesized and characterized using mass spectrometry. The estimated reduction potential of cysteine disulfides of synthetic peptides varied from -298 to -328 mV, similar to the active site cysteine disulfide motifs of the redox family of proteins.

Conformational change due to replacement of disulfide with selenosulfide and diselenide in dipeptide vicinal cysteine loop.

Replacement of disulfide bridges with diselenide bridges is increasingly common to improve the stability, foldability, and structural refinement of the cysteine-rich polypeptides. Even though the global structural features are similar due to the replacement of disulfide with diselenide, the local conformational differences have been reported in a few polypeptides. The current report has used the constrained vicinal cysteine disulfide as the model to access the influence of the replacement of disulfides with diselenide on the local conformations.

High lipid accumulating bacteria isolated from dairy effluent scum grown on dairy wastewater as potential biodiesel feedstock.

The study evaluated the lipid accumulation potential of bacteria isolated from dairy effluent scum by the valorization of dairy wastewater as a renewable feedstock for biodiesel production. Three oleaginous bacteria (i.e.

Performance and kinetic study of semi-dry thermophilic anaerobic digestion of organic fraction of municipal solid waste.

Anaerobic digestion (AD) of the organic fraction of municipal solid waste (OFMSW) is promoted as an energy source and waste disposal. In this study semi dry anaerobic digestion of organic solid wastes was conducted for 45 days in a lab-scale batch experiment for total solid concentration of 100g/L for investigating the start-up performances under thermophilic condition (50 °C). The performance of the reactor was evaluated by measuring the daily biogas production and calculating the degradation of total solids and the total volatile solids.