Differential Effect of Simulated Microgravity on the Cellular Uptake of Small Molecules.

The space environment can affect the function of all physiological systems, including the properties of cell membranes. Our goal in this study was to explore the effect of simulated microgravity (SMG) on the cellular uptake of small molecules based on reported microgravity-induced changes in membrane properties. SMG was applied to cultured cells using a random-positioning machine for up to three hours.

Implementing a Photodocumentation Program.

The widespread availability of smart devices has facilitated the use of medical photography, yet photodocumentation workflows are seldom implemented in healthcare organizations due to integration challenges with electronic health records (EHR) and standard clinical workflows. This manuscript details the implementation of a comprehensive photodocumentation workflow across all phases of care at a large healthcare organization, emphasizing efficiency and patient safety. From November 2018 to December 2023, healthcare workers at our institution uploaded nearly 32,000 photodocuments spanning 54 medical specialties.

Higher chronic absenteeism threatens academic recovery from the COVID-19 pandemic.

The broad and substantial educational harm caused by the COVID-19 pandemic has motivated large federal, state, and local investments in academic recovery. However, the success of these efforts depends in part on students' regular school attendance. Using state-level data, I show that the rate of chronic absenteeism among US public-school students grew substantially as students returned to in-person instruction.

A community response approach to mental health and substance abuse crises reduced crime.

Police officers often serve as first responders to mental health and substance abuse crises. Concerns over the unintended consequences and high costs associated with this approach have motivated emergency response models that augment or completely remove police involvement. However, there is little causal evidence evaluating these programs.

Ethnic studies increases longer-run academic engagement and attainment.

Increased interest in anti-racist education has motivated the rapidly growing but politically contentious adoption of ethnic studies (ES) courses in US public schools. A long-standing rationale for ES courses is that their emphasis on culturally relevant and critically engaged content (e.g.

The gift of time? School starting age and mental health.

Using linked Danish survey and register data, we estimate the causal effect of age at kindergarten entry on mental health. Danish children are supposed to enter kindergarten in the calendar year in which they turn 6 years. In a "fuzzy" regression-discontinuity design based on this rule and exact dates of birth, we find that a 1-year delay in kindergarten entry dramatically reduces inattention/hyperactivity at age 7 (effect size = -0.

Do parental involvement laws deter risky teen sex?

Parental involvement (PI) laws require that physicians notify or obtain consent from a parent(s) of a minor seeking an abortion before performing the procedure. Several studies suggest that PI laws curb risky sexual behavior because teens realize that they would be compelled to discuss a subsequent pregnancy with a parent. We show that prior evidence based on gonorrhea rates overlooked the frequent under-reporting of gonorrhea by race and ethnicity, and present new evidence on the effects of PI laws using more current data on the prevalence of gonorrhea and data that are novel to this literature (i.

A prospective investigation of the relationship between child maltreatment and indicators of adult psychological well-being.

The study of psychological well-being will advance understanding of child maltreatment effects and resilience processes. In this study, the mean level of anger in adulthood was significantly higher for those identified three decades earlier as having been maltreated. Mean levels of self-esteem, autonomy, purpose in life, perceived (fewer) constraints, and happiness and satisfaction were lower for those who were maltreated according to child welfare reports.

Metal removal via particulate material in a lowland river system.

Twelve month surveys of acid-soluble and dissolved trace metal concentrations in the lower Waikato River (in 1998/9 and 2005/6) showed abnormally low particulate Fe, Mn, Cu, Pb and Zn concentrations and mass flux in autumn, when the suspended particulate material (SPM) had a relatively high diatom and organic carbon content, and low Fe and Al content. Dissolved Mn, Cu and Zn concentrations also decreased in autumn, while dissolved Fe and Pb concentrations were unaffected. While SPM settlement under the low river flow conditions present in autumn can explain the removal of particulate metals, it does not explain dissolved metal removal.

Motorcycle helmets and traffic safety.

Between 1997 and 2005, the number of annual motorcyclist fatalities doubled. Motorcyclist fatalities now account for over 10 percent of all traffic-related fatalities. However, over the last three decades, states have generally been eliminating laws that require helmet use among all motorcyclists.

The strength of graduated drivers license programs and fatalities among teen drivers and passengers.

Objectives: The purpose of this study is to investigate the effects of differentially stringent graduated drivers license programs on teen driver fatalities, day-time and night-time teen driver fatalities, fatalities of teen drivers with passengers present, and fatalities among teen passengers.

Methods: The study uses 1992-2002 data on motor vehicle fatalities among 15-17-year-old drivers from the Fatality Analysis Reporting System to identify the effects of "good", "fair", and "marginal" GDL programs based upon designations by the Insurance Institute for Highway Safety. Analysis is conducted using conditional negative binomial regressions with fixed effects.

Graduated driver licensing and teen traffic fatalities.

Over the last 8 years, nearly every state has introduced graduated driver licensing (GDL) for teens. These new licensing procedures require teen drivers to advance through distinct stages where they are subject to a variety of restrictions (e.g.

AIDS mortality may have contributed to the decline in syphilis rates in the United States in the 1990s.

Background: The mortality associated with AIDS among men may have had an influence on primary and secondary syphilis trends among men in the United States, through the loss of men at high risk for acquisition or transmission of syphilis in this population and/or by prompting safer sexual behaviors in response to the threat of AIDS.

Goal: The goal of this study was to examine the association between AIDS mortality rates and primary and secondary syphilis incidence rates among men in the United States from 1984 to 1997.

Study Design: We used a fixed-effects regression analysis of state-level AIDS mortality rates and primary and secondary syphilis incidence rates for men.

Alcohol abuse and economic conditions: evidence from repeated cross-sections of individual-level data.

This study presents novel evidence on the relationship between macroeconomic conditions and patterns of alcohol consumption. Prior research has suggested that alcohol abuse varies procyclically, implying that income effects dominate any drinking patterns related to the opportunity cost of time or the psychological stress of recessions. However, those inferences have been based either on aggregate measures of consumption volume or possibly confounded cross-sectional identification strategies.

The complementarity of teen smoking and drinking.

Teen drinkers are over twice as likely as abstainers to smoke cigarettes. This empirical study provides evidence of a robust complementarity between these health behaviors by exploiting the "cross-price" effects. The results indicate that the movement away from minimum legal drinking ages of 18 reduced teen smoking participation by 3 to 5%.

Reconsidering the effects of seat belt laws and their enforcement status.

The debate over the benefits of mandatory seat belt laws and their enforcement status has focused on a controversial empirical enigma: why have these policies, which appear to have increased belt use sharply, had a relatively small impact on traffic fatalities? In this paper, I offer new insights into this question by examining panel data on observed belt use from the National Highway Traffic Safety Administration and self-reported data on belt use from pooled cross-sections of the Centers for Disease Control and Prevention's 1985-1993 Behavioral Risk Factor Surveillance System. By exploiting the panel nature of these data, I demonstrate that prior estimates, which have not conditioned on the unobserved time-varying determinants of belt use, have dramatically overestimated the impact of seat belt laws and their enforcement status on belt use. The true effects are more consistent with the modest impact these policies have had on traffic fatalities without having to appeal to the possibility of risk compensation by drivers.

Propionibacterium avidum as the etiologic agent of splenic abscess.

Propionibacterium avidum was isolated from an intrasplenic abscess in a patient recovering from coronary artery bypass surgery. This organism has not previously been reported as an etiologic agent of splenic abscess nor has splenic abscess been described as a complication of coronary bypass surgery. This report emphasizes the potential pathogenicity of normal microbial flora following surgical manipulation.

Sensitivity, specificity, and predictive value of anti-candida serum precipitin and agglutinin quantification: comparison of counterimmunoelectrophoresis and latex agglutination.

Many serological techniques have been developed to aid in the discrimination of significant candidiasis from other clinical states. Serum anti-candida precipitin and agglutinin quantification by counterimmunoelectrophoresis and latex agglutination were statistically evaluated as to their respective ability to accomplish this discrimination. Forty-five serum specimens from 16 patients with documented disease and 2 with presumptive disease and 81 specimens from 70 control patients were studied.

Alterations in serum opsonic activity and complement levels in pneumococcal disease.

Pneumococcal opsonic activity and concentrations of pneumococcal capsular polysaccharide antigen, C3, C4 factor B, C3 and factor B breakdown products were measured in the serum obtained acutely from 12 patients with serious pneumococcal disease. One patient showed markedly reduced pneumococcal opsonic activity, borderline-low C3, and the presence of C3 and factor B breakdown products and died. Although eight additional patients showed depressed levels of C3 or C4 or the presence of C3 or factor B breakdown products, none had reduced pneumococcal opsonic activity.

Stability of anti-Candida precipitating antibody in stored sera as determined by counterimmunoelectrophoresis.

Prolonged storage of sera at -20 degrees C did not significantly affect anti-Candida precipitin titers measured by counterimmunoelectrophoresis when compared with the original determinations.

Correlation of antistaphylococcal antibody titers with severity of staphylococcal disease.

Counterimmunoelectrophoresis (CIE) was utilized to determine antistaphylococcal precipitin antibody titers in patients with various staphylococcal diseases and in control subjects. Patients with staphylococcal disease comprised five cases of endocarditis, 22 of deep tissue infection (including seven cases of osteomyelitis), six of bacteremia and six of skin infection. Control subjects consisted of 31 patients with nonstaphylococcal bacteremias, 29 hospitalized patients without infection and 30 healthy subjects.

Gentamicin and tobramycin penetration into synovial fluid.

The penetration of gentamicin and tobramycin into the synovial fluid of 12 patients with nontraumatic joint effusions was studied. Simultaneous serum and synovial fluid specimens taken after the intramuscular or intravenous administration of an antimicrobial agent were assayed. Synovial fluid levels of antibiotic were >50% of serum levels in all subjects studied.

Immunologic studies in pneumococcal disease.

Many patients die from pneumococcal disease despite the availability of effective antimicrobial agents. Immunologic studies including detection, typing, and quantitation of serum pneumococcal capsular polysaccharide (PCP) antigen by counterimmunoelectrophoresis (CIE), quantitation of PCP antibody by radioimmunoassay (RIA), and quantitation of serum complement components C3, C4, and C3PA and serum immunoglobulins IgG, IgM, and IgA by the radial immunodiffusion technique of Mancini were performed with the sera of 18 patients. Five patients died (group I), and 13 survived (group II) pneumococcal infection.

Detection of candida serum precipitins by counterimmunoelectrophoresis: an adjunct in determining significant candidiasis.

We report our experience with the use of counterimmunoelectrophoresis for the detection and quantitation of candida serum precipitins in 164 patients. Group I consisted of 24 patients with significant candidiasis; group II consisted of 97 patients with either colonization or transient candidemia; and group III consisted of 43 subjects with noncandida systemic mycoses, bacterial infections, and normal controls. Prospective studies were done in ten patients.