Publications by authors named "Declan Kohl"
Rapid detection of protein and small-molecule analytes is a valuable technique across multiple disciplines, but most in vitro testing of biological or environmental samples requires long, laborious processes and trained personnel in laboratory settings, leading to long wait times for results and high expenses. Fusion of recognition with reporter elements has been introduced to detection methods such as enzyme-linked immunoassays (ELISA), with enzyme-conjugated secondary antibodies removing one of the many incubation and wash steps. Chimeric protein switch biosensors go further and provide a platform for homogenous mix-and-read assays where long wash and incubation steps are eradicated from the process.
View Article and Find Full Text PDFEnzyme - Switch sensors for therapeutic drug monitoring of immunotherapies.
Biosens Bioelectron
October 2023
Article Synopsis
- Therapeutic monoclonal antibodies (TmAb) are effective treatments for cancers and autoimmune diseases, but their effectiveness can vary greatly between patients, making therapeutic drug monitoring (TDM) important for dosage optimization.
- This study presents a novel enzyme switch sensor platform, combining β-lactamase with custom binding proteins, to rapidly and sensitively measure the levels of two specific TmAbs, trastuzumab and ipilimumab, in blood serum.
- While the sensor successfully detected these two antibodies at low concentrations suitable for therapy, it struggled with other antibodies, highlighting its potential for bedside monitoring and personalized treatment adjustments.
Objectives: Penicillin allergy records are often incorrect and may result in harm. We aimed to systematically review the effectiveness and safety of nonallergist health care worker delivery of penicillin allergy delabeling.
Methods: We searched EMBASE/MEDLINE/CINAHL (Ovid), PsycInfo, Web of Science, and Cochrane CENTRAL from inception to January 21, 2022 and unpublished studies and gray literature.
Objective: This review will systematically examine and synthesize the evidence evaluating the effectiveness and safety of interventions that enable non-allergy specialist health care workers to assess allergy risk in patients with reported penicillin allergies and subsequently remove erroneous allergy records.
Introduction: The potential benefits of removing erroneous penicillin allergy labels (de-labeling) are wide-ranging. Penicillin allergy assessment and de-labeling is an antibiotic stewardship priority.