Publications by authors named "Deborah de Melo Magalhaes Padilha"
Article Synopsis
- - The study focuses on isolating and characterizing oviduct fluid extracellular vesicles (coEV) in goats, which are important for events around conception, as they have been previously identified in other mammals but not in caprine species.
- - Two experiments were conducted: one to isolate coEV from individual goats or a pool of three, and the other to analyze coEV during different phases of the estrous cycle (follicular vs. luteal).
- - Findings showed that while the size distribution of coEV was similar regardless of isolation method, those collected during the luteal phase were larger, and the presence of specific proteins indicated they were exosomes with distinct characteristics.
Objective: Medical students are especially vulnerable to situations of poor sleep quality due to academic demands. The COVID-19 pandemic brought significant changes and high psychological stress, causing a great impact on this population. Here we aim to analyze the influence of the pandemic on the sleep quality of medical students.
View Article and Find Full Text PDFThis study was conducted to characterize the morphology and morphometry of follicles containing multiple oocytes (MOFs) and determine the association with the FecG mutation in Santa Inês ewes. Based on the genotypes, 65 ewes were characterized as being homozygous wild-type (n = 25; FecG), heterozygous mutant (n = 27, FecG), and homozygous mutant (n = 13, FecG). The variables evaluated were follicle developmental stage, number of oocytes per follicle, morphology, and morphometry of MOFs.
View Article and Find Full Text PDFThis study evaluated the effects of bioidentical nanostructured progesterone alone or in association with human chorionic gonadotropin (hCG) on the in vitro survival and development of preantral follicles (experiment 1) and oocyte maturation (experiment 2). Bioidentical hormones have a molecular structure identical with that of endogenous hormones; nanostructured substances refer to those reduced to a nanoscale. In experiment 1, fragments of goat ovarian tissue were cultured for 7 days in α-MEM alone or supplemented with nanoprogesterone (MEM + P4) or P4 and hCG (MEM + P4 + hCG).
View Article and Find Full Text PDFEvaluation of in vitro culture systems for goat preantral follicles using reused ovaries from reproductive biotechniques: An alternative to maximize the potential of reproduction.
Reprod Domest Anim
March 2019
This study aimed to examine the in vitro culture of secondary preantral follicles, using reused ovaries, to compare both the 2D and 3D methods of in vitro culture of preantral follicles, and the system of medium replacement. Twenty-five pairs of ovaries from mixed-breed goats were used for the experiment. Follicular puncture of antral follicles was performed for in vitro production.
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