Publications by authors named "Deborah V Hoyle"
O26 is the commonest non-O157 Shiga toxin ()-producing serogroup reported in human infections worldwide. Ruminants, particularly cattle, are the primary reservoir source for human infection. In this study, we compared the whole genomes and virulence profiles of O26:H11 strains ( = 99) isolated from Scottish cattle with strains from human infections ( = 96) held by the Scottish O157/STEC Reference Laboratory, isolated between 2002 and 2020.
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- Integrons are genetic elements that enhance the spread of antimicrobial resistance genes in bacteria, and this study focused on their prevalence in Scottish cattle across different farms.
- Researchers analyzed fecal samples from 108 cattle herds and found high prevalence rates for class 1 and 2 integrons but did not detect class 3.
- Factors influencing integron presence included seasonal effects, with spring showing higher positivity, and management practices like using natural spring water for cattle and housing conditions at sampling time.
Article Synopsis
- Cattle in Scotland serve as a reservoir for Shiga toxin-producing (STEC) pathogens, contributing to higher rates of STEC infections among humans compared to the European average.
- A study collected and analyzed fecal samples from 110 herds, revealing varying herd-level prevalence rates for non-O157 serogroups O26, O103, O111, and O145, with O26 being the most common.
- Seasonal and regional differences were noted, with higher prevalence of certain serogroups in the South West during autumn, and a lack of positive herds associated with Central Scotland and winter.
Fever is one of the most common reasons for seeking health care globally and most human pathogens are zoonotic. We conducted a systematic review to describe the occurrence and distribution of zoonotic causes of human febrile illness reported in malaria endemic countries. We included data from 53 (48·2%) of 110 malaria endemic countries and 244 articles that described diagnosis of 30 zoonoses in febrile people.
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- The study found that 11% of bovine E. coli contained Shiga toxin genes, but none of the human isolates tested positive for these toxins.
- Though many bovine strains showed Shiga toxin presence, they mostly belonged to non-threatening serogroups, suggesting that while current strains are unlikely to cause human disease, there's still a risk for future virulent strains to emerge.
Background: Escherichia coli (E. coli) O157 is a virulent zoonotic strain of enterohaemorrhagic E. coli.
View Article and Find Full Text PDFObjectives: The aim of this study was to identify the profile of antibiotic resistance among E. coli O26, O103 and O145 in two cohorts of Scottish beef cattle on two farms and to determine whether there is an association between resistant phenotypes and the genotypic PFGE patterns to suggest clonality among resistant strains.
Methods: MICs of 11 antibiotics for 297 E.
Pulsed-field gel electrophoresis (PFGE) was used to investigate the dissemination and diversity of ampicillin-resistant (Amp(r)) and nalidixic acid-resistant (Nal(r)) commensal Escherichia coli strains in a cohort of 48 newborn calves. Calves were sampled weekly from birth for up to 21 weeks and a single resistant isolate selected from positive samples for genotyping and further phenotypic characterization. The Amp(r) population showed the greatest diversity, with a total of 56 different genotype patterns identified, of which 5 predominated, while the Nal(r) population appeared to be largely clonal, with over 97% of isolates belonging to just two different PFGE patterns.
View Article and Find Full Text PDFAge-related decline in carriage of ampicillin-resistant Escherichia coli in young calves.
Appl Environ Microbiol
November 2004
The presence of ampicillin-resistant Escherichia coli (Amp(r) E. coli) in the fecal flora of calves was monitored on a monthly basis in seven cohorts of calves. Calves were rapidly colonized by Amp(r) E.
View Article and Find Full Text PDFObjectives: The acquisition of antibiotic-resistant commensal Escherichia coli was examined in a cohort of newborn calves.
Methods: Faecal samples were collected weekly from calves over a 4 month period and screened for E. coli resistant to ampicillin, apramycin and nalidixic acid at concentrations of 16, 8 and 8 mg/L, respectively.