Publications by authors named "Deborah McCombs"
Article Synopsis
- Microgravity conditions, utilized since the early 1980s, enhance protein crystallization by minimizing convection and creating a sedimentation-free environment, leading to higher quality crystals.
- Experiments aboard the International Space Station (ISS) have shown that although crystal growth rates are slower in microgravity, this environment allows for extended, detailed studies on macromolecular transport and impurity incorporation in growing crystals.
- Findings reveal that crystals grown in microgravity incorporate fewer impurities and often display better quality, providing insights for predicting which proteins might benefit most from this crystallization method.
Structure of nicotinic acid mononucleotide adenylyltransferase from Bacillus anthracis.
Acta Crystallogr Sect F Struct Biol Cryst Commun
October 2008
Nicotinic acid mononucleotide adenylyltransferase (NaMNAT; EC 2.7.7.
View Article and Find Full Text PDFPreliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p.
Acta Crystallogr Sect F Struct Biol Cryst Commun
February 2006
Article Synopsis
- Protein translocations across mitochondrial membranes are essential for the formation of mitochondria, involving transport from the cytosol to the mitochondrial matrix through TOM and TIM complexes.
- Tim44p is a crucial mitochondrial protein that is part of the TIM23 translocon, facilitating the delivery of mitochondrial protein precursors.
- Researchers have successfully crystallized yeast Tim44p, achieving diffraction at 3.2 A, and are currently working on determining its structure using MAD methods.