Publications by authors named "Deborah D Skinner"
Microbiology (Reading)
September 1999
Article Synopsis
- The study reports the cloning of acdH genes from Streptomyces coelicolor and Streptomyces avermitilis, which encode an enzyme involved in breaking down branched-chain amino acids.
- Both acdH genes showed strong similarity to acyl-CoA dehydrogenases from various organisms, and when expressed in E. coli, the proteins could oxidize different acyl-CoA derivatives.
- A mutation in the acdH gene of S. avermitilis hindered its growth on certain amino acids and affected the incorporation of specific labeled carbon sources into avermectin, confirming the gene's crucial role in amino acid metabolism.
