Performance of self-etching adhesives on caries-affected primary dentin treated with glutaraldehyde or silver diamine fluoride.

Objectives: to evaluate the quality and stability of adhesive interfaces established by self-etching adhesives on caries-affected primary dentin (CAD) treated with glutaraldehyde (GA) or silver diamine fluoride (SDF).

Methods: 42 primary molars were exposed to a microbiological caries-inducing protocol and divided into 6 groups according to the adhesive system (Clearfil SE - CL or FL Bond II - FL) and pretreatment (water, GA or SDF) applied on CAD. One tooth from each group was analyzed for surface modification using infrared spectroscopy.

Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation.

Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation.

Methods: Dentin beams (5 × 2 × 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens.

Proteolytic activity and degradation of bovine versus human dentin matrices.

Objective: Non-human teeth have been commonly used in research as replacements for human teeth, and potential dissimilarities between the dental tissues should be considered when interpreting the outcomes. To compare the proteolytic activity and degradation rate of bovine and human dentin matrices.

Methodology: Dentin beam specimens were obtained from human molars (n=30) and bovine incisors (n=30).

Response of pulp cells to resin infiltration of enamel white spot-like lesions.

Objectives: To investigate the trans-enamel and trans-dentinal biological effects of treating enamel white spot-like lesions (EWSLs) with resin infiltration components (RICs) on odontoblast-like cells (MDPC-23) and human dental pulp cells (HDPCs).

Methods: EWSLs were induced in 60 enamel/dentin discs (4.0 ± 0.

COVID-19 pandemic and pediatric dentistry: Fear, eating habits and parent's oral health perceptions.

Background: Severe measures have been implemented around the world to reduce COVID-19 spread with a significant impact on family dynamics.

Aim: To assess the impact of the pandemic on fear, dietary choices and oral health perceptions of parents.

Design: questionnaire containing 19 questions was remotely applied to 1003 parents of children aged 0-12 years.

Proteolytic activity, degradation, and dissolution of primary and permanent teeth.

Background: Primary and permanent teeth composition may influence dissolution and degradation rates.

Aim: To compare the dissolution and degradation of primary and permanent teeth.

Design: Enamel and dentin powders were obtained from primary molars and premolars and incubated within different pH buffers.

Effect of Er:YAG laser irradiation and chitosan biomodification on the stability of resin/demineralized bovine dentin bond.

Objective: The purpose of this study was to evaluate the effect of Er:YAG laser for selective removal of carious lesion, followed by biomodification with chitosan on the microtensile bond strength (µTBS), adhesive interface, dry mass loss and hydroxyproline release (HYP).

Methods: Artificial lesions were created in 104 bovine dentin blocks. Blocks were divided according to caries removal method: bur or Er:YAG laser.

Effect of crosslinkers on bond strength stability of fiber posts to root canal dentin and in situ proteolytic activity.

Statement Of Problem: Improved stability of the adhesive interface can be obtained using crosslinkers. However, research on the use of crosslinkers in root dentin is lacking.

Purpose: The purpose of this in vitro study was to evaluate the effect of crosslinkers on the proteolytic activity of root dentin and on the bond strength of resin-cemented fiber posts.

Cross-linked dry bonding: A new etch-and-rinse technique.

Objective: To determine if acid-etched, cross-linked dentin can be dehydrated without lowering bond strength below that of cross-linked wet-bonded dentin in vitro.

Methods: Using extracted human third molars, control acid-etched dentin was bonded with Single Bond Plus, using either the wet- or dry-bonding technique. Experimental acid-etched dentin was treated with 5mass% grape seed extract (GSE) in different solvents for 1min before undergoing wet vs dry resin-dentin bonding with Single Bond Plus.

Biocompatibility of a restorative resin-modified glass ionomer cement applied in very deep cavities prepared in human teeth.

This study evaluated whether a restorative resin-modified glass ionomer cement, Vitremer (VM), would be biocompatible with pulp tissue when used as a liner in very deep cavities prepared in young human permanent teeth. Two dental cements in current use as liner materials, Vitrebond (VB) and Dycal (DY), were compared to VM. Class V cavities were prepared in 36 sound premolars that were scheduled for extraction, and the cavity floor was lined with the restorative cement (VM) or a liner/base control cement (VB or DY).

Effects of a Dicalcium and Tetracalcium Phosphate-Based Desensitizer on In Vitro Dentin Permeability.

The present study evaluated the effectiveness of a dicalcium and tetracalcium phosphate-based desensitizer in reducing dentin permeability in vitro. Dentin fluid flow was measured before and after treatment of dentin with patent dentinal tubules using 1 or 3 applications of the dicalcium and tetracalcium phosphate containing agent TeethmateTM (TM) and comparing the results with two sodium fluoride varnishes VellaTM (VLA) and VanishTM (VAN), after storage in artificial saliva for 24 h, 48 h and 7 days. Significant differences were observed among the 4 methods employed for reducing dentin permeability (p < 0.

Transdentinal cytotoxicity of glutaraldehyde on odontoblast-like cells.

Objectives: This study investigated the transdentinal cytotoxicity of glutahaldehyde-containing solutions/materials on odontoblast-like cells.

Methods: Dentin discs were adapted to artificial pulp chambers. MDPC-23 cells were seeded on the pulpal side of the discs and the occlusal surface was treated with the following solutions: water, 2% glutaraldehyde (GA), 5% GA, 10% GA, Gluma Comfort Bond+Desensitizer (GCB+De) or Gluma Desensitizer (GDe).

Immediate human pulp response to ethanol-wet bonding technique.

Objectives: To evaluate the short-term response of human pulps to ethanol-wet bonding technique.

Methods: Deep class V cavities were prepared on 17 sound premolars and divided into three groups. After acid-etching, the cavities from groups 1 (G1) and 2 (G2) were filled with 100% ethanol or distilled water, respectively, for 60 s before the application of Single Bond 2.

Effect of time between adhesive application and photoactivation on adhesion and collagen exposure.

Purpose: To evaluate the influence of the time elapsed between the application and photoactivation of Single Bond 2 (SB) on microtensile bond strength (μTBS) and collagen exposure at the adhesive interface produced in the presence of intrapulp pressure.

Methods: Dentin occlusal surfaces were prepared in 72 molars, and divided into eight groups (n = 9). After acid etching, SB was applied, with or without simulated intrapulp pressure, and remained undisturbed for 0, 20, 40 or 60 seconds, before photoactivation.

Water distribution in dentin matrices: bound vs. unbound water.

Objective: This work measured the amount of bound versus unbound water in completely-demineralized dentin.

Methods: Dentin beams prepared from extracted human teeth were completely demineralized, rinsed and dried to constant mass. They were rehydrated in 41% relative humidity (RH), while gravimetrically measuring their mass increase until the first plateau was reached at 0.

Esthetic dental anomalies as motive for bullying in schoolchildren.

Facial esthetics, including oral esthetics, can severely affect children's quality-of-life, causing physical, social and psychological impairment. Children and adolescents with esthetic-related dental malformations are potential targets for bullies. This study was aimed to present and discuss patients who suffered from bullying at school and family environment due to esthetic-related teeth anomalies.

Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques.

Objective: The general aim of this article is to describe the state-of-the-art of biocompatibility testing for dental materials, and present new strategies for improving operative dentistry techniques and the biocompatibility of dental materials as they relate to their interaction with the dentin-pulp complex.

Methods: The literature was reviewed focusing on articles related to biocompatibilty testing, the dentin-pulp complex and new strategies and materials for operative dentistry. For this purpose, the PubMed database as well as 118 articles published in English from 1939 to 2014 were searched.

Inhibition of endogenous human dentin MMPs by Gluma.

Objective: The objective of this study was to determine if Gluma dentin desensitizer (5.0% glutaraldehyde and 35% HEMA in water) can inhibit the endogenous MMPs of dentin matrices in 60 s and to evaluate its effect on dentin matrix stiffness and dry mass weight.

Methods: Dentin beams of 2 mm×1 mm×6 mm were obtained from extracted human third molars coronal dentin.

Effect of reducing acid etching time on bond strength to noncarious and caries-affected primary and permanent dentin.

Purpose: The purpose was to evaluate the effect of acid etching time on the bond strength of a simplified etch-and-rinse adhesive system to noncarious and caries-affected dentin of primary and permanent teeth.

Methods: Twenty-four extracted primary and permanent teeth were divided into three groups, according to the acid etching time. Four teeth from each group were exposed to a microbiological caries-inducing protocol.

Stabilization of dentin matrix after cross-linking treatments, in vitro.

Objectives: To evaluate the effect of EDC on elastic modulus (E), MMPs activity, hydroxyproline (HYP) release and thermal denaturation temperature of demineralized dentin collagen.

Methods: Dentin beams were obtained from human molars and completely demineralized in 10 wt% H3PO4 for 18 h. The initial E and MMP activity were determined with three-point bending and microcolorimetric assay, respectively.

Effect of acid etching time on demineralization of primary and permanent coronal dentin.

Purpose: To determine the effect of acid etching time on dentin calcium solubilization and to compare the solubilization rate of primary and permanent dentin when etched for the same period of time.

Methods: Flat coronal dentin surfaces were produced on primary (n=8) and permanent (n=8) sound teeth. Three 2 mm-diameter areas were delimited on each surface and assigned to 5, 10 or 15 seconds of acid etching.